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potravinárstvo<br />
coagulation in the presence of CaCl2 (Pęksa, <strong>2006</strong>). The<br />
Table 1 Formulations for pellets producing<br />
Formulations Potato<br />
starch<br />
(g/100 g)<br />
1<br />
(Control-0)<br />
2<br />
(Control-PPC)<br />
Potato<br />
semolina<br />
(g/100 g)<br />
composition of mentioned above PPC was as follows:<br />
3.20 % moisture content, 72.73 % crude protein, 1.45 %<br />
fat, 7.25 % ash and 15.37 % carbohydrates.<br />
Preparation of samples<br />
The formulations of pellets are shown in Table 1. Two<br />
levels of fiber products (wheat bran–WB, corn bran–CB<br />
and corn germ–CG) mixed with potato starch, potato<br />
semolina and other ingredients were formed. Potato starch<br />
was replaced, in suitable quantity, by fiber product. One<br />
level of potato protein concentrate (PPC) was added to<br />
mixtures on the basis of sample weight. As controls,<br />
samples without any additives or containing only PPC as<br />
an additive, there were used. Duplicate batches (600g<br />
each), (fourteen samples in each) were moisten up to<br />
40-45 %, mixed and sieved. The samples were placed in<br />
sealed polyethylene bags at 4-5 o C for 12 hours to<br />
equilibrate moisture.<br />
Preparation of pellets<br />
Material for pellets was extruded in a single-screw<br />
extruder (Brabender DN 20, Germany). The following<br />
extrusion parameters were kept constant: die size (0,5mm<br />
Other<br />
ingredients<br />
(g/100 g)<br />
WB/CB/CG<br />
(g/100 g) (%)<br />
67 26 7 0 0<br />
67 26 7 0 3<br />
3 62 26 7 5 0<br />
4 57 26 7 10 0<br />
5 62 26 7 5 3<br />
6 57 26 7 10 3<br />
x 80mm), speed of dough supplier (38 rpm), screw speed<br />
(120 rpm), screw loading (2.8 A), barrel temperature<br />
distribution (60 o C / 70 o C / 80 o C). Previously subjected to<br />
extrusion, not expanded strands were cut into pellets of ca.<br />
27 x 27 mm and dried at room temperature for 12 hours to<br />
ca. 12-13 % of moisture content and then sealed in<br />
polyethylene bags to equilibrate moisture until frying.<br />
Preparation of fried snacks<br />
Snacks were obtained from pellets after 48 hours of<br />
storing in room temperature. Samples were fried in hot<br />
(180 o C) rapeseed oil for 15-20 seconds (3 seconds after<br />
expanded product appears on the oil surface). The product<br />
to oil ratio was kept at 1:20 w/v.<br />
Analysis<br />
Proximate chemical analysis: Standard AOAC methods<br />
(1975) were used for the analysis of fat, total ash and the<br />
nitrogen content in raw material components and snacks<br />
and the crude protein content was calculated as Nx6.25.<br />
The moisture content of the ingredients for pellets<br />
Table 2. Mean values of the properties of snacks fortified with different levels of fiber<br />
Supplements.<br />
Fiber<br />
supplement<br />
Level of<br />
fortification<br />
[%]<br />
Moisture<br />
content<br />
[%]<br />
Fat<br />
content<br />
[%]<br />
Expansion<br />
Bulk<br />
density<br />
[g/ml]<br />
Texture<br />
[N]<br />
0<br />
3,22<br />
Wheat bran<br />
(WB)<br />
5<br />
10<br />
a<br />
3,37 a<br />
2,96 a<br />
27,55 c<br />
32,50 b<br />
36,70 a<br />
2,96 a<br />
2,55 a<br />
2,19 a<br />
0,185 c<br />
0,223 b<br />
0,282 a<br />
14,9 a<br />
11,2 a<br />
8,3 a<br />
Corn bran<br />
(CB)<br />
Corn germ<br />
X (mean )<br />
0<br />
5<br />
10<br />
X (mean)<br />
0<br />
5<br />
10<br />
3,17 A<br />
3,22 a<br />
3,73 a<br />
3,55 a<br />
3,64 A<br />
3,22 a<br />
3,47 a<br />
3,18 a<br />
32,25 A<br />
27,55 b<br />
32,45 a<br />
31,90 a<br />
30,63 B<br />
ročník 4 60 2/2010<br />
2,56 A<br />
2,96 a<br />
2,73 a<br />
2,26 a<br />
2,65 A<br />
0,230 A<br />
0,185 a<br />
0,148 b<br />
0,163 a<br />
0,165 B<br />
11,5 B<br />
14,9 a<br />
13,2 a<br />
14,2 a<br />
14,1 AB<br />
PPC<br />
Lightness<br />
[L value]<br />
71,84 a<br />
58,16 b<br />
51,81 c<br />
60,60 C<br />
71,84 a<br />
60,51 b<br />
61,24 b<br />
64,53 B<br />
X (mean)<br />
a,b,c – Means in the same column with different letters differ significantly (P≤ 0,05) by Duncan’s multiple<br />
range test<br />
A,B,C - Means in the same column with different capital letters differ significantly (P≤ 0,05) by Duncan’s<br />
multiple range test<br />
3,33 A<br />
27,55 c<br />
34,85 b<br />
37,35 a<br />
33,25 A<br />
2,96 a<br />
2,86 a<br />
2,23 a<br />
2,68 A<br />
0,185 b<br />
0,275 a<br />
0,272 a<br />
0,244 A<br />
14,9 b<br />
22,8 a<br />
13,9 b<br />
17,2 A<br />
71,84 c<br />
76,48 a<br />
73,96 b<br />
74,09 A