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Kontaktná adresa:<br />
Ing. Bronislava Škarbová, PhD. Ministerstvo<br />
pôdohospodárstva SR, Dobrovičova 12, 812 66 Bratislava,<br />
tel.: 02 566 402, e-mail: bronislava.skarbova@land.gov.sk<br />
doc. Ing. Jozef Golian, Dr., Slovenská poľnohospodárska<br />
univerzita v Nitre, FBP, KHBP, Trieda Andreja Hlinku 3, Email:<br />
jozef.golian.af@uniag.sk<br />
NIGROSPORA – NOVÝ ENDOFYT NA PŠENICI (TRITICUM AESTIVUM L.)<br />
SLOVENSKÉHO PÔVODU<br />
NIGROSPORA – NEW ENDOPHYTE FROM WHEAT (TRITICUM AESTIVUM L.)<br />
OF SLOVAK ORIGIN<br />
Dana Tančinová, Zuzana Mašková, Soňa Felšöciová, Mária Dovičičová, Zuzana Barboráková,<br />
Roman Labuda<br />
ABSTRACT<br />
The aim of this study was to investigate endogenous contamination of wheat (Triticum aestivum L.) grains with focus on<br />
new endophytic filamentous fungi – Nigrospora. A total of 52 wheat samples were analyzed. They were harvested from<br />
different regions of Slovakia in seasons <strong>2006</strong> (21 samples), 2007 (30 samples) and 2008 (21 samples). The endogenous<br />
contamination was detected by direct plating method on Dichloran Rose Bengal Chloramphenicol agar (DRBC) after<br />
superficial sterilization of grains. Total endogenous contamination by micromycetes was ranging from 26 to 100 %. This<br />
study discovered only 1 species - Nigrospora oryzae, which was detected in 47.62 % (season <strong>2006</strong>), 60.00 % (season 2007)<br />
and 28.57 % (season 2008) of wheat samples. This species was presented at 1.54 % (season <strong>2006</strong>), 4.97 % (season 2007)<br />
and 0.86 % (season 2008) of analyzed wheat grains.<br />
Keywords: wheat, endogenous contamination, Nigrospora<br />
ročník 4 94 1/2010
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