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1/2006 - Potravinárstvo

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multifunctional metabolic pathway in a marine mollusk leads<br />

to unprecedented prostaglandin derivatives (prostaglandin<br />

1,15-lactones), Biochem. J. 273, 593-600<br />

GARDNER, H. W. 1991. Recent investigations into the<br />

lipoxygenase pathway of plants, Biochim. Biophys. Acta,<br />

1084, 221-39<br />

GERWICK, W. H. 1993. Carbocyclic oxylipins of marine<br />

origin, Chem. Rev., 93, 1807-1823<br />

GUERRIERO, A. D., AMBROSIO, M., PIETRA, F. 1990.<br />

Novel Hydroxyicosatetraenoic and Hydroxyicosapentaenoic<br />

Acids and a 13-Oxo Analog. Isolation from a Mixture of the<br />

Calcareous Red Algae Lithothamnion corallioides and<br />

Lithothamnion calcareum of Brittany Waters, Helv. Chim.<br />

Acta 73, 2183-2189<br />

HADAS, E. 1988. Biosynthesis of prostaglandins in<br />

pathogenic and nonpathogenic strains of Acanthamoeba<br />

castellani, Acta Protozool., 27, 61- 65<br />

ICHIRO, N., KAZUHIKO, S., MASAHISA, T. 1991.<br />

Methods for producing prostaglandins and for purifying the<br />

same, European Patent 0424915<br />

ICHIRO, N., KAZUHIKO, S., MASAHISA, T. 1992.<br />

Purification of prostaglandins from the far east seaweed<br />

Gracilaria verrucosa using nonpolar porous resins, Japan<br />

Patent 04112866<br />

LAMAČKA, M, ŠAJBIDOR, J. 1995. The occurrence of<br />

prostaglandins and related compounds in lower organisms,<br />

Prostaglandins Leukot Essent Fatty Acids 52, 357-64<br />

ÚVOD<br />

Kvasinky sú celosvetovo najpoužívanejšie priemyselné<br />

mikroorganizmy, ktoré majú široké uplatnenie v<br />

potravinárskych technológiách a vo farmaceutickom<br />

priemysle. Mnohé sú využívané ako biologické modelové<br />

systémy pre štúdium metabolizmu eukaryotických buniek.<br />

Sú ľahko manipulovateľné na molekulovej úrovni, a preto<br />

sa využívajú aj pre produkciu rekombinantných proteínov<br />

(Wang, Chen, <strong>2006</strong>).<br />

Využitie kvasiniek je zaujímavé z nutričného hľadiska,<br />

kvôli zloženiu ich bunkovej hmoty. Chemické zloženie<br />

<strong>Potravinárstvo</strong><br />

LAMAČKA, M., ŠAJBIDOR, J. 1998. The content of<br />

prostaglandins and their precursors in Mortierella and<br />

Cunninghamella species, Lett Appl Microbiol., 26, 224-226<br />

MIYAOKA, H., HARA, Y., SHINOHARA, I.,<br />

KUROKAWA, T., YAMADA, Y. 2005. Synthesis and<br />

structural revision of marine eicosanoid agardhilactone,<br />

Tetrahedron Lett., 46, 7945-7949<br />

PACE - ASCIAK, C. R. 1989. Mass spectra of<br />

prostaglandins and related compounds. Advances in<br />

Prostaglandin, Thromboxane and Leukotriene Research,<br />

Raven Press, New York, p. 565<br />

SCHERA, J. U., PILLINGERA, M. H. 2005. 15d-PGJ 2. The<br />

anti-inflammatory prostaglandin? Clinical Immunol., 114,<br />

100-109<br />

SCHNEIDER C., MANIER, M. L., HACHEY, D. L,<br />

BRASH, A. R. 2002. Detection of the 15-acetate of<br />

prostaglandin E2 methyl ester as a prominent component of<br />

the prostaglandins in the gorgonian coral Plexaura<br />

homomalla, Lipids 37, 217-21<br />

Poďakovanie: Práca bola podporená grantom APVV 0310-<br />

06<br />

Adresa: Ján Šajbidor, Fakulta chemickej a potravinárskej<br />

technológie STU v Bratislave, Radlinského 9, 812 37<br />

Bratislava, jan.sajbidor@stuba.sk<br />

CHEMICKÁ A BIOCHEMICKÁ ANALÝZA KVASINIEK SACCHAROMYCES<br />

CEREVISIAE KMEŇ KOLÍN, 612 A GÖYNG<br />

CHEMICAL AND BIOCHEMICAL ANALYSIS OF YEAST SACCHAROMYCES<br />

CEREVISIAE STRAIN KÖLN, 612 AND GÖYNG<br />

Silvia Šillerová, Anežka Poláková, Dana Urminská, Eva Szabová<br />

ABSTRACT<br />

In this study three strains of Saccharomyces cerevisiae Köln, 612 and Gyöng were analysed. The experiment focused on<br />

chemical and biochemical analysis of yeasts. The strain Köln contained the highest amount of proteins (49 %) and lipids<br />

(5.88 %). It was found that the strain Gyöng contains up to 1.5 % saccharides. Vitamin B2 is the most widely occurring<br />

vitamin in these strains with the highest content noted in strain Köln (0.02 %). Analysis also showed that microelements<br />

such as Zn, Fe, Cu and Mn are the most prevalent in the strain Köln, too. As a result, it may be stated that composition of<br />

yeasts may be a reason for their use in feed and food industries.<br />

Keywords: Chemical composition, yeast, Saccharomyces cerevisiae, biomass, analysis<br />

kvasiniek je do značnej miery ovplyvňované zmenami<br />

v zložení kultivačného média a podmienkami kultivácie.<br />

Podľa viacerých autorov (Swanson, Fahey, 2004; Milić et<br />

al., 2007) sú však základné charakteristiky sušiny<br />

kvasiniek rovnaké vo vysokom obsahu bielkovín,<br />

sacharidov a vitamínov, kým koncentrácia tukov<br />

a minerálnych látok je relatívne nízka.<br />

Podiel bielkovín a sacharidov sa dá ovplyvniť obsahom<br />

dusíkatých živín v živnom médiu. Albers et al., (2007)<br />

pozorovali pri nedostatku dusíka, ale v prítomnosti<br />

glukózy v živnom médiu, akumuláciu sacharidov a tukov,<br />

ročník 4 85 1/2010

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