TThe PCR pproducts werre digested eendonuclease RRsa I (Segenetic) in the to µ µl at 37 ºC ovvernight accoording to the innstructions. TThe productss of restrictio separated eleectrophoreticcally on 4 (MMerck) and were docummented by D (BBioRad). Inn the case oof presence oof allele A, w ddetection of aallele C as a subtype of wwas used thee method off RFLP-PCR ccomposition of reaction mixture as ddetection off alleles A and B, eexchange(figuure 1). The pprimers used aallele C were: fo forward primer: (5'- TCAGGACCCCG AAAC-3') reverse primeer: ( 5'- CCTTCCAGCTG AAAG-3') TThe temperature profile of the PC stated the samme, besides tthe temperatu thhat was maiintained at 663 ºC. PCR ddigested by restriction eendonuclease tootal volume of 20 µl at 337 ºC overni thhe manufactuurer’s instruuctions and s aagarose gel. FFrom the obbtained results, the freq ggenotype annd allelic ppresence w FFrequency hoomogeneity iin the individ ppopulations wwas evaluateed with χ FFalconer et MMackay (1996) SStatistical annalysis were SSTATISTICA 6.0 (StatS aalgorithms, whhich are avai mmilk protein ccomposition w thhe least squaares method G 2 with restricction otal volume oof 20 e manufacturrer’s on analysis wwere % agarose gel DigiDoc Sysstem we have madde a f allele A. Thhere R with the same in the casee of with primmers for detection of GGAGGTGGGAC GGTCGGGTTTG R analysis was ure of anneaaling fragments wwere e Msp I in the ght accordinng to separated in 4 % quencies of the were calculaated. dually examiined test accordinng to ). e carried oout in proggram Soft, Inc. PPL, 2001) used ilable on requuest. The dataa on were analysedd statistically with GLM (Generaal Linear Model), TTable 1: Distrribution of β-Lg genotypess and gene frrequencies in Improved Vaalachian, Cziggaia and East-Friesian sheeep bbreeds. Allele Genoty ypic frequeencies fr requency bbreed n AA AB BB χ22 test A B obs. 22 34 19 IImproved VValachian sheep 75 exp. 20.28 37.44 17.28 0. .633 0.5 52 0.48 nn=number of ssamples, χ2 teest=chi-squareed value, obs. =observed fre equencies, expp.=expected ffrequencies ca alculated on thhe bbasis of Hardyy-Weinberg laww TThe genotypicc frequencies at the β-Lg loocus estimated by RRFLP-PCR, ddetected for thhe Improved Valachian shheep wwere 0.29 (AAA); 0.45 (AB) ); 0.26 (BB). In our study, the vvariant A of ββ-Lg was moore frequent in the exammined ppopulation of sheep breed tthan variant BB. It corresponnded wwith the findinngs of severaal authors tessting the diffeerent oovine breeds aas Altamuranaa (Dario et all., 2005), Masssese (RRampolli et al.,1997), Litthuanian Natiive Coarsewoooled aand Lithuaniaan Blackface sheep (Kuččinskiene et al., 22005), Hungarrian merino ett British milkk sheep (Antoon et aal., 1997), Maanchega breedd (Lopez-Gallvez et al., 1994) aand the majority of studied oovine breeds. ročník 4 Pootravinárst tvo 60 taki ing into accouunt the effects of genotype es of ß-LG annd sam mpling. The ddifferences weere tested by y using Schefffe mul ltiple range teest, LSD test aand Student te est for all traiits stud died. M – 25 bp markeer, 1 – AA geenotype, 2 – AB A genotype, 3 – BB genotype, 4 – PCR prodduct. Figure 1: The rrepresentativee results of the t RFLP-PCCR anal lysis. RE ESULTS ANND DISCUSSSION We estimated thhe distributionn of β-Lg ge enotypes in thhe pop pulation of thee Slovak sheepp breed Impro oved Valachiaan. The e alleles A annd B were preesent in exam mined breed, oon the contrary, alllele C was nnot found. The T allelic annd gen notypic frequeencies in the exon 2 of β-Lg gene aare show wn in table 1. In the t tested bre diff ference in χ obs dist con equ The sign dist only case vs usin 2 eed, there wass found out th he insignificaant 2 test calculaated between expected annd erved genotyype frequenciies. Accordin ng to an ideeal tribution of thhe genotypes in the popu ulation, we caan nfirm that thhe populationn was in Hardy-Weinber H rg uilibrium. e Improved VValachian sheeep showed no statistically nificant effectt of the geneetic variant fo or the nitrogeen tribution in thhe proteins (taable 2). We have h found thhe y statistical ddifference (P<
the effect of the lactation period on the crude protein nitrogen, true protein nitrogen, casein and casein number (figure2) (P
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Vedecký èasopis pre potravinárst
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ÚVOD Pšenica je celosvetovo jedna
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oqueforti (2), P. carneum (1), P. c
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aminokyselín a ión-párového či
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Tab. 2b: pokračovanie. Literatura
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- Page 13 and 14: Pri ostatných hodnotených ukazova
- Page 15 and 16: Potravinárstvo positive significan
- Page 17 and 18: REFERENCE AYADI, M., CAJA, G., SUCH
- Page 19 and 20: zastúpení a stanoviť optimálny
- Page 21 and 22: ZÁVER Jačmeň nahý sa vyznačuje
- Page 23 and 24: Bielkovinové frakcie (albumíny, g
- Page 25 and 26: detegovaná podjednotka 2+12, ktor
- Page 27 and 28: PAYNE, P. I., NIGHTINGALE, M. A., K
- Page 29 and 30: different copies of integrated gene
- Page 31 and 32: INTRODUCTION Flaxseed and walnut oi
- Page 33 and 34: Table 1. Characteristics of fresh c
- Page 35 and 36: ∆E 18 16 14 12 10 8 6 4 2 0 Potra
- Page 37 and 38: ÚVOD Mlieko je vzhľadom na svoje
- Page 39 and 40: log K T J .m l -1 Obrázok 2: Prež
- Page 41 and 42: ÚVOD Kvasinky a vláknité huby s
- Page 43 and 44: Okrem uvedených metabolitov môžu
- Page 45 and 46: Obbr. 2: Vplyv kkultúry Lactobbaci
- Page 47 and 48: ÚVOD V súčasnosti medzi najdôle
- Page 49 and 50: Index klíčenia Index klíčenia 1
- Page 51 and 52: ZÁVER Pozberové dozrievanie (dorm
- Page 53 and 54: Potravinárstvo Table 1: The summar
- Page 55 and 56: period. Żyw. Człow. Metab., 2007,
- Page 57 and 58: pH vzoriek bol tiež zaznamenaný.
- Page 59 and 60: DISKUSIA Testovanie vplyvu tepla a
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- Page 65 and 66: CONCLUSION We have confirmed the in
- Page 67 and 68: 15 % sušiny (Dawczynski et al., 20
- Page 69 and 70: ylo do filtračních sáčků (F 57
- Page 71 and 72: metodami in vitro, kdy je možné s
- Page 73 and 74: parametry byly stanoveny jednak met
- Page 75 and 76: ČURDA, L., KUKAČKOVÁ, O., NOVOTN
- Page 77 and 78: Zdroj žiarenia: γ-žiarenie z Co
- Page 79 and 80: celková chutnosť vôňa doznievan
- Page 81 and 82: predstavuje len veľmi miernu hladi
- Page 83 and 84: prítomnosti kvasiniek a plesní, n
- Page 85 and 86: Potravinárstvo Tab. 1: (pokračova
- Page 87 and 88: multifunctional metabolic pathway i
- Page 89 and 90: Kolín a naopak najnižšie hodnoty
- Page 91 and 92: ZÁVER Analýza získanej biomasy k
- Page 93 and 94: produkciu potravín živočíšneho
- Page 95 and 96: Vypočítané hodnoty iónových po
- Page 97 and 98: ÚVOD Rod Nigrospora Zimmerm. podľ
- Page 99 and 100: Potravinárstvo Obrázok 1: Nigrosp
- Page 101 and 102: LITERATÚRA CLEAR, R. M., PATRICK,
- Page 103 and 104: vlastnosť pri priemyselne vyrobeno
- Page 105 and 106: Potravinárstvo Obrázok 1: Závisl
- Page 107 and 108: práce a typ múky sú faktory ktor
- Page 109 and 110: ICC STANDARD NO. 106/2, 1984. Worki
- Page 111 and 112: ZUZANA BARBORÁKOVÁ, SOŇA FELŠÖ
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