OPTICKÉ BIOSENZORY.pdf - FBMI
OPTICKÉ BIOSENZORY.pdf - FBMI
OPTICKÉ BIOSENZORY.pdf - FBMI
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Optické biosenzory- OWLS<br />
OWLS – Optical Waveguide Lightmode<br />
Spectroscopy (Brusatori – ITO film,<br />
10 nm, coated onto SrTiO<br />
waveguding film, serves as the<br />
working sensing electrode.<br />
Adsorption is detected by monitoring<br />
changes in the effective refractive<br />
index of the waveguide- ITO film<br />
plus adsorbed layer (indium tin<br />
oxide).<br />
Fig. 1. A schematic of the ITO coated OW 2400<br />
sensor chip (Micro Vacuum Ltd., Budapest).<br />
Fig. . A schematic of the sensor chip as a four-layer<br />
optical waveguide. Monochromatic light penetrates a<br />
glass substrate (S) at an angle and is coupled into a<br />
waveguiding film (F) via a grating. A second film (F′),<br />
representing either a conducting layer or adsorbed<br />
species, may separate the waveguiding layer from the<br />
cover (C) solution. A guided mode is detected when the<br />
phase shift of one complete period of total internal<br />
reflection equals an integral multiple of 2 . The guided<br />
mode is characterized by propagation angles F and F′.
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