CamSep 1
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Rys. 5. Porównanie rozdzielania peptydów na kolumnie aminowej (I) i DIOL (II) z innymi dodatkami<br />
do eluentów [23] Kolumna I - TSK gel Amide-80 250x4.6 mm, Kolumna II – TSK gel OH-<br />
120 250x4,6 mm; Eluent: (A) A - AcCN-H 2 O 97:3 + 0,1% TFA, B – AcCN-H 2 O 55:45 + 0.1%<br />
TFA (B) A - AcCN-H 2 O 97:3 + 0.1% TFA+TEA, B – AcCN-H 2 O 55:45 + 0.1% TFA+TEA;<br />
(C) A - AcCN-H 2 O 97:3 + 0.2% TFA+TEA, B – AcCN-H 2 O 55:45 + 0.2% TFA+TEA; Program<br />
elucji: 70 min liniowo gradient H 2 O od 3 to 45% (0.6% H 2 O/min), T=40 0 C, v=1.0 ml/min.<br />
Detekcja UV 215 nm; 1 - FY, 2-FGGF, 3 - FLEEI, 4 - DYMGWMDP-NH 2 , 5 - NFTYGGF,<br />
6 - AGSE, 7 - WAGGDASGE, 8 - YGGFMTSQKSQTPLVT, 9 - ASTTTNYT,<br />
10 - VLSEGEWQLVLHVWAKVEADVAGHGQDILI-RLFKSHPETLEKFDRFKHLKTEAEM *)<br />
( * )M-homoserine)<br />
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