electrophoretic analysis is present in to somatomedins and was shown toFig 6.influence cartilage metabolism.Concluding presented results it can beDiscussionstressed that chondrocytes have the abilityThe presence of aggrecan in culture to form constructs of cartilage in vitro aftermedium was substantial for formation of beeing seeded onto scaffold material.scaffold by cartilage collagens. Growth may be achieved in usual tissueInteractions between the cell and the ECM conditions and enhanced using theare critical to a number of biological tripeptide GHK (2). Supplementation of<strong>pro</strong>cesses that take place in tissues like culture media with aggrecan and thecartilage. These <strong>pro</strong>cesses include cell tripeptide GHK was critical for preservinganchorage, growth, differentiation, chondrocytes phenotype and stimulatingmigration, as well as matrix synthesis and chondrocyte <strong>pro</strong>liferation. This studydegradation. The attachment is mediated by indicated that tissue engineering has theintegrins and is down regulated by potential for succesful repair of articularenzymatic release of chondrocytes from cartilage defects.cartilage (18). However it is up regulatedduring monolayer culture and the Referencesattachment of chondrocytes mainly with 1. Adam M, Kühn K. Investigations on thecollagen (type II) and aggrecan is very reaction of metals with collagen in vivo. 1.important not only because of matrix Comparison of the reaction of goldsynthesis but also because of chondrocytes thiosulphate. Eur J biochem, 3, 1968,phenotype <strong>pro</strong>tection (2). Therefore we 407-11.substituted culture medium with aggrecan. 2. Benya PD, Shaffer J D. DedifferentiatedWe have used cartilage collagen to prepare chondrocytes reexpress the differentiatedconstructs as it is well known that presence collagen phenotype when cultured inof collagen type I or III accelerates agarose gels. Cell, 30, 1982, 215-24.dedifferentiation of chondrocytes (17). 3. Brittberg M, Nilsson A, Lundahl A,In the case when the defects were Ohlsson C, Paterson L. Rabbit articulardrilled through the subchondral bone the cartilage deffects treated with autologousmechanism of repair appeared to be cultured. Clin Orthop, 326, 1996, 270-mediated rather by the <strong>pro</strong>liferation and 83.differentiation of mesenchymal cells of the 4. Farndale R W, Buttle D J, Barrett A J.marrow, than by chondrocytes of the I m p r o v e d q u a n t i f i c a t i o n a n dimplant, i.e. mainly fibrocartilage was d i s c r i m i n a t i o n o f s u l f a t e dformed.glycosaminoglycans by use of dimethyleneIn fact the major role of implants is to blue. Biochim Biophys Acta, 883, 1986,stimulate ingrowth of chondrocytes from 173 -77.original cartilage, to preserve their 5. Grande D A, Pitman M I, Peterson L,phenotype and to up regulate their Menche D, Klein M. The repair ofsynthetic activity (15). We prefered the use experimentally <strong>pro</strong>duced defects inof tissues ap<strong>pro</strong>priate aggrecan to agarose rabbit articular cartilage by autologous(16) or alginates (19). Modulation of chondrocyte transplantation. J Orthopwound healing in the case of grafts involves Res., 7, 1989, 208-18.release of growth factors, one of them is 6. Green W T J. Behaviour of articularcartilage-derived growth factor, which like chondrocytes in cell culture. Clinfibroblast growth factor is peptide similar Orthopaed Related Res, 75, 1971, 1109 -40LOCOMOTOR SYSTEM VOL. 7, <strong>2000</strong>, No.1
41. 911-15.7. Hascall V C, Sajdera S W. 15. Pešáková V, Štol M, Gillery P,Proteinpolysaccharide complex from Maquart F X, Borel J P, Adam M. Thebovine nasal cartilage. A comparison of effect of different c o l l a g e n s a n dlow and high shear extractive <strong>pro</strong>cedures. J <strong>pro</strong>teoglycan on the retraction ofBiol Chem, 244, 1969, 2384 -95. c o l l a g e n l a t t i c e . B i o m e d a n d8. Holtzer H, Abbott J, Lash J, Holtzer S. Pharmacother, 48, 1994, 261- 266.The loss of phenotypic traits by 16. Rahfoth B, Weisser J, Sternkopf F.differentiated cells in vitro. Proc Natl Transplantation of allograft chondrocytesAcad Sci, 46, 1960, 1533 - 1537. embedded in agarose gel into cartilage9. Kimura T, Yasui N, Ohsawa S, Ono K. defects of rabbits. Osteoarthritis andChondrocytes embedded in collagen Cartilage, 6, 1998, 50 -65.gels maintain cartilage phenotype during 17. Robinson P D. Articular cartilage of thelong-term cultures. Clin Orthoped.,. 186, temporomandibular joint: Can it1984, 231-39. regenerate? Ann R Coll Surg Engl, 75,10. Klagsburn M. Large-scale preparation 1993, 2316 - 2319.of chondrocytes. Methods Enzymol, 58, 18. Sommarin Y, Larsson T, Heinegard1979, 560 - 565. D. Chondrocyte-matrix interactions:11. Laemmli K. Cleavage of structural attachment to <strong>pro</strong>teins isolated from<strong>pro</strong>teins during the assembly of the head of cartilage. Exp Cell Res, 184, 1989, 181bacteriophage T4. Nature (London), -92.227, 1970, 680 -88. 19. Van Susante J L C, Buma P, van Osch12. Manning W. Bonner W. Isolation and G J V M. Culture of chondrocytes inculture of chondrocytes from human alginate and collagen carrier gels. Actaadult articular cartilage. Arthritis Orthop Scand 66, 1995, 549-56.Rheum, 10, 1967, 235 - 39.20. Wirth C J, Rudert M. Techniques of1 3 . M i l l e r E . I s o l a t i o n a n d c artilage growth enhancement: a reviewcharacterisation of collagen from of the literature. Arthroscopy, 12, 1996,chick cartilage containing three identical 300 - 08.đ-chains. Biochemistry, 10, 1971,1652-59.14. Pešáková V, Novotná J, Adam M. Effectof the tripeptide glycyl-L-histidyl-L- Address:lysine-L on the p r o l i f e r a t i o n a n d Prof. MUDr. M..Adam, DrSc.synthetic activity of chick embryo Revmatologický ústavchondrocytes. Biomaterials., 16, 1995, Na Slupi 4, 128 00 Praha 2POHYBOVÉ ÚSTROJÍ, ročník 7, <strong>2000</strong>, č. 1 41
- Page 1 and 2: POHYBOVÉ ÚSTROJÍročník 7, 2000
- Page 3 and 4: POHYBOVÉ LOCOMOTORÚSTROJÍSYSTEM1
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- Page 19 and 20: Fig. 9a-c. Trauma of the thumb in a
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- Page 31 and 32: Tabulka č.2. Hladiny sledovaných
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