1/2000 - Společnost pro pojivové tkáně

electrophoresis: collagen of a part ofexplanted newly formed tissues fromcartilage defects was extracted by digestionwith pepsin and characterised withpolyacrylamide gel electrophoresis, usingcontinuous buffer system according toLaemmli (11).ResultsAccording to electron microscopy ofSLS forms the length of isolated collagen IImolecules varied between 40 and 50 nm.Chromatography of isolated aggrecan isshown in Graph 1. Macroscopically thedefects were almost completely filledafter nine weeks after surgery (Fig 2).About the same could be seen in X-ray Graph 1.pictures. Molecular sieve chromatography ofaggrecan on Sepharose 4B (90 x 0.95cm), elution and equilibration buffer:0.5 sodium acetate, pH 7.0, roomtemperature, flow rate: 11.0ml/ hour,absorbance 238 nm, glycosaminoglycanscontent measured in 20 min. fractionsaccording to Farndale et al., 1986.Histology of implants showed somevariations within space-filling constructs,nevertheless in all samples healingprocesses were observed. Newly formedcartilage is thicker than the original one.Cellularity of the tissue is rather high andisogenetic clusters of chondrocytes arepresent (Figs 3 and 3a). Formation ofECM containing aggrecans starts on thesurface of subchondral bone and proceedsupwards (Fig 4). When defect was drilledthrough the subchondral bone (Figs 5and 5a) a central area of the defect,near join cavity is fulfilled with fibrotictissue, however the walls of the defectwere covered with thick, rich onFig.2. Photography of femoral part of knee aggrecans, cartilage layer.joint 9 weeks after surgery, in the Electrophoretic analysis showed in allintercondylic region is defect with samples the presence of collagen type II,implant.on the other hand collagen types I and IIIwere not present. Typical example ofPOHYBOVÉ ÚSTROJÍ, ročník 7, 2000, č. 1 37