1/2000 - Společnost pro pojivové tkáně

implanted into the cartilage defect, it is Material and methods (2.)conceived that the microenviromental 2.1. Composition of the constructconditions will lead to cell expansion and 2.1.1. Collagen : after pepsinization ofnew cartilage tissue formation. Collagen calf articular cartilage, cartilagetype II and especially collagen - graft - collagens (types II, IX, XI) were isolated.glycosaminoglycan copolymers is an C o l l a g e n w a s p u r i f i e d b yexcellent biomaterial for chondrocytes chromatography on DEAE-celluloseembedding. They dramatically modify the (DE-52 Whatman, Clifton, NJ) (13).inflammatory response and lead to the Length of isolated collagen moleculessynthesis of physiological tissue was measured by means of SLS forms(regeneration) rather than to scar tissue using electron microscope (1).formation. In a previous paper we studied 2.1.2. Aggrecan: (hyaline cartilagethree dimensional chondrocyte cultures proteoglycan) was extracted from calf(15). As we were able to culture articular cartilage with 4 M guanidiniumchondrocytes without any change in their hydrochloride and desalted on Biogelphenotype, we used those conditions in this P2, 200-400 mesh (Bio-Rad Laboratories,study to develop constructs consisting of California) (7). To characterise isolatedautologous three dimensional cultured aggrecan it was dissolved in 0.5 Mc h o n d r o c y t e s , c o l l a g e n t y p e I I sodium acetate (pH 7.0) and(cartilaginous), and proteoglycan to chromatographed on 4B Sepharoseregenerate damaged articular cartilage. (Pharmacia Fine Chemicals, Sweden).Fig.1. Diagram of implant preparation and transplantation inthe femorale condyle.POHYBOVÉ ÚSTROJÍ, ročník 7, 2000, č. 135