1/2000 - Společnost pro pojivové tkáně

PŮVODNÍ PRÁCE * ORIGINAL PAPERTRANSPLANTATIONOF AUTOLOGOUS CHONDROCYTESINTO CARTILAGE DEFECTS OF MINIPIGS1) 2) 3) 1) 1)Pešáková V., Klézl Z., Čech O., Pohunková H., Adam M.1)Institute for Rheumatology, Prague2)Orthopaedic - Traumatology Dept., Central Military Hospital, Prague3)Orthopaedic Clinic, III. Med.Fac., Charles Univ., Prague, Czech RepublicSummaryDurcupan.Pešáková V, Klézl Z, Čech O, Results: the defects were almostP o h u n k o v á H , A d a m M . completely filled after nine weeks afterTransplantation of autologous surgery. Newly formed cartilage waschondrocytes into cartilage defects of thicker than the original one. Cellularity ofminipigs.the tissue was rather high and isogeneticRunning title: chondrocytesclusters of chondrocytes were present.transplantation Formation of ECM started on theObjective: to prepare a cartilage implant surface of subchondral bone andthe choice of a matrix for the proceeded upwards.chondrocytes embedding is very Key words: chondrocytes, cartilageimportant because chondrocytes have collagens, aggrecan, the tripeptide Glyextremelylabile phenotype. Our previous His-Lys.study (14) showed that aggrecan is criticalfor a scaffold formation. Collagen-graft- Introductionglycosaminoglycan copolymers (CGGC) The grafting of cells derived from adramatically modify the inflammatory cartilage into defect sites is currently veryresponse and lead to the synthesis of popular (9,5,20,3). Since the 1960´sphysiological tissue rather than to scar chondrocytes following enzymatic releasetissue formation.may be cultured in vitro (8,12,10). VeryDesign: 22 minipigs one year old were important determinant of the outcome isused to study the effect of construct the choice of matrix within which cells arecomposed from cartilage collagens, embedded. The main problem of theaggrecan, the tripeptide Gly-His-Lys, and cartilage replacement is namely extremeautologous chondrocytes.lability of chyndrocyte phenotype. TheseSurgery: cartilage explants from the cells very rapidly change their shape andlateral condyle were the source of phenotypic expression under inappropriatechondrocytes. 2-3 weeks later an artificial conditions. They then produce collagendefect was performed with a trephine in types I and III instead of collagen type II,the intercondylic region of the left knee, that means that fibrillar instead of hyalinewhich was filled with the construct. cartilage is formed. However, fibrillarHistology: newly formed tissue was cartilage has some physiologicaleither fixed in formalin for cryostat disadvantages in the comparison to thesections or embedded into epoxy resin - hyaline one. When chondrocytes are34LOCOMOTOR SYSTEM VOL. 7, 2000, No.1