Sborník příspěvků z workshopu MICROMYCO 2008 - Ústav půdní ...

The first records of entomopathogenic fungi Beauvaria bassiana and Isaria
fumosorosea (syn. Paecilomyces fumosoroseus) on the hibernating pupae of
the horse chestnut leaf-miner Cameraria ohridella (Lepidoptera:
Gracillariidae)
EVA PRENEROVÁ, ROSTISLAV ZEMEK, FRANTIŠEK WEYDA
PRENEROVÁ, E., ZEMEK, R., WEYDA, F. - The first records of entomopathogenic fungi Beauvaria bassiana
and Isaria fumosorosea (syn. Paecilomyces fumosoroseus) on the hibernating pupae of the horse chestnut
leaf-miner Cameraria ohridella (Lepidoptera: Gracillariidae)
Proceedings from the MICROMYCO 2008, Nováková, A. (Ed.): p. 79. ISB BC AS CR, v.v.i., 2008. ISBN
978-80-86525-12-9
Cameraria ohridella Deschka & Dimic, 1986 (Lepidoptera, Gracillariidae) is an important invasive pest in
Europe attacking mainly a horse chestnut, Aesculus hippocastanum. Since its first record in Macedonia in 1985 it
rapidly spread and colonised major parts of Europe including Denmark, south of Sweden, Belorussia and
Ukraine. The larvae feed on leaf parenchyma and at high population density the damages can result in the tree
growth reduction. Present methods of its control are based on application of non-selective insecticides and
composting or burning of leaf litter. Since these methods also kill beneficial organisms including natural enemies
of C. ohridella, new environment-friendly approaches need to be developed. While most studies published so far
have focused on parasitoids and predators like beetles, spiders and birds attacking leaf-mining larvae and pupae,
our project is aimed at entomopathogenic fungi as potential candidates for biological control of this pest. In the
present work we report the first records of Beauveria bassiana (Bals.-Criv.) Vuill. and Isaria fumosorosea Wize
(Deuteromycetes) as entomopathogenic fungi of C. ohridella. Both species were found and isolated from
hibernating pupae of C. ohridella. Samples of the horse chestnut leaves with diapausing C. ohridella pupae were
collected in autumn at České Budějovice, South Bohemia, the Czech Republic (49 ºN). The leaves were
dissected and the pupae were individually placed into the 23°C and 95% R.H. Development of mycosis on pupae
was frequently observed but most were saprophytic species. Only a few pupae were covered by mycelia with
conidiophores and conidia of entomopathogenic fungi. The strains B. bassiana and I. fumosorosea were isolated
from individual infected pupae and deposited in CCEFO (Culture Collection of Entomopathogenic Fungi
Olesna) in the Czech Republic. The virulence of these strains is being evaluated.
This work was supported by the Ministry of Education, Youth and Sports (grant No. 2B06005).
Keywords: horse chestnut leaf-miner, Cameraria ohridella, entomopathogenic fungi, biological control,
mycoinsecticide, Isaria fumosorosea, Beauveria bassiana, Deuteromycetes.
Eva Prenerová, Laboratory of Plant Protection Olešna, Olešna 87, Bernartice u Milevska, Czech Republic. E-
mail: eva.prenerova@seznam.cz
Rostislav Zemek, František Weyda, Institute of Entomology, Biology Centre AS CR, Branišovska 31, Česke
Budejovice, Czech Republic. E-mail: rosta@entu.cas.cz, weyda@entu.cas.cz
∗ Presented at workshop MICROMYCO 2008, České Budějovice, Czech Republic, September 2-3, 2008.
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