Optické mikroskopy

The quality of a photomicrograph, either digital or recorded on film, is dependent upon the quality of
the microscopy. Film is a stern judge of how good the microscopy has been prior to capturing the
image. It is essential that the microscope be configured using Köhler illumination, and that the field
and condenser diaphragms are adjusted correctly and the condenser height is optimized. When
properly adjusted, the microscope will yield images that have even illumination over the entire field of
view and display the best compromise of contrast and resolution.
Almost all microscopists will, at some point, have the need or desire to record the images seen
through the microscope. The main mechanism, for many years, of
producing such photomicrographs
was through the use of film, although in recent years most scientists have begun to capture images by
means of electronic cameras. The main purpose of this tutorial is to enable the microscopist to record
the observed images on film or digital media, and to do so with accuracy of image reproduction and
with fidelity of color when color film is being used. The further aim is to empower the
photomicrographer to secure excellent pictures without having to struggle through the already existing,
far more complex reference literature. Use the links below to navigate to various topics in our
discussions of photomicrography.
Troubleshooting Problems in Photomicrography - Photography through the microscope is
undergoing a transition from film to digital imaging. New digital technologies are producing higher
resolution micrographs, but the quality still falls short of that obtainable with film. Microscope
configuration errors represent the greatest obstacle to quality photomicrographs, followed by errors
in
filter selection, film choice, aberration, dirt and debris, and processing mistakes.
Adresa: http://www.biomed.cas.cz/d331/vade/mikroskopy.html
8. Mikroskopy
1. Optický mikroskop
2.Zobrazení buněk v kultuře mikroskopem invertovaným
3. Zobrazení buněk v tkáňových řezech mikroskopem přímým
4. Zeiss a Olympus
5. Zvětšení mikroskopu
6. Numerická aperura objektivu
7. Koehlerovo osvětlení
8. Fázový kontrast
9. Nomarského diferenciální interferenční
kontrast (DIC)
10. Hoffmanův modulační kontrast (HMC)
11. Dodtův infračervený
gradientový kontrast (Dodt gradient contrast (DGC)
2. Fluoresnční mikroskop
2. Epifluorescence
3. Monochromátor
4. Videomokroskopie
3. Konfokální mikroskop
4. Pojmy
1. Optický mikroskop
2. Zobrazení buněk v kultuře mikroskopem invertovaným
3. Zobrazení buněk v tkáňových řezech mikroskopem přímým
4. Zeiss a Olym pus
5. Zvětšení mikroskopu
6. Numerická apertura objetivu
7. Koehlerovo osvětlen
8. Fázový kontrast
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