Graz˙yna Budryn, Ewa Nebesny FENOLOKWASY – ICH WŁAS ...

194 R. Amarowicz
Nr 2
WNIOSKI
Hydroliza n-butanolem/HCl uwalnia z proantocyjanidyn obecnych w gryce
i kaszy gryczanej cyjanidyne˛ oraz dwie inne antocyjanidyny róz˙ne od delfinidyny
i malwinidyny. Zastosowana metoda moz˙e być stosowana w badaniach gryki
i kaszy gryczanej jako składników z˙ywnos´ci funkcjonalnej.
R. A m a r o w i c z
HPLC ANALYSIS OF ANTHOCYANIDINS AFTER HYDROLYSIS
OF PROANTHOCYANIDINS FOUND IN EXTRACTS FROM BUCKWHEAT
AND BUCKWHEAT GROATS
Summary
Extracts of buckwheat seeds and roasted buckwheat groats were prepared using 80% acetone.
Proanthocyanidins from the extracts were hydrolysed using n-butanol/HCl. The released anthocyanidis
were analysed by HPLC. Peaks from cyanidin and two additional unknown compounds A and B were
noted on the chromatograms. The spectra of the latter two were characterised by maxima at 510, 421,
320, 255 nm (compound A) and 510, 416, 324, 254 nm (compound B). The total contents of
anthocyanidins in extracts of buckwheat seeds and buckwheat groats were 3.26 mg/g and 2.73 mg/g,
respectively.
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