Zborník príspevkov z vedeckej konferencie - Department of ...

CONCLUSIONS
This work studied the possibilities of preparative isotachophoresis (pITP) as a sample pretreatment technique before
high performance liquid chromatography-mass spectrometric separation (HPLC-MS) in analysis of high molecular weight
compounds present in complex biological matrix. The analysis of chosen analyte (human lysozyme) present in complex
biological matrix (saliva) at different concentration levels was selected as an example of solving the analytical problem by
using pITP-HPLC-MS combination. Without using pITP pretreatment it was impossible to detect lysozyme in saliva by
direct HPLC-MS analysis while its using significantly simplified the saliva sample what is favourable for the minimizing of
the matrix effects in HPLC-MS. Human lysozyme was found in the saliva samples of 4 volunteers using just deconvolution
of data from MS mode after the background correction. Proper optimization of HPLC separation conditions as well as the
data acquisition in MS and MS/MS modes can improve both the level of identification and the concentration limit of
detection of lysozyme present in biological matrices.
Acknowledgements
This work was supported by a grant from the Slovak Research and Development Agency (No.VVCE-0070-07) and the grants
of Slovak Grant Agency, No`s.1/0882/09 and 1/0546/10.
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Zborník príspevkov
z 18. medzinárodnej vedeckej konferencie
"Analytické metódy a zdravie loveka", ISBN 978-80-969435-7-9
- 132 -
hotel Falkensteiner, Bratislava
11. - 14. 10. 2010