Zborník príspevkov z vedeckej konferencie - Department of ...

lysozyme
TE
TRIS
CREA
HIS
GABA
EACA
TE
Fig.3: Isotachopherogram obtained from the pITP Fig.4: Schema of fractionation procedure.
analysis of model mixture of discrete spacers. The
arrow indicates the migration position of lysozyme.
The migration position of lysozyme was checked by the HPLC-MS analyses of the 6 fractions obtained during the pITP
fractionation of lysozyme standard. It was proved that lysozyme is migrating only between TRIS and HIS spacers (2nd
fraction) what is declared in Fig.5 where TIC traces and MS spectra of the 1st, 2nd and 3rd fractions are shown.
The HPLC-MS analyses of the first 3 fractions obtained during the pITP fractionation of saliva sample are shown in
Fig.6. As can be seen, there is small peak of human lysozyme visible in the deconvoluted averaged and background corrected
MS spectrum from the 2nd fraction while no peak of lysozyme is present in the 1st and the 3rd fractions. The presence of
lysozyme in the saliva sample was also proved by the spiking of the saliva sample with the lysozyme standard what is shown
in Fig.7 where TIC traces and MS spectra of the 1st, 2nd and 3rd fractions obtained from such samples are shown.
Zborník príspevkov
z 18. medzinárodnej vedeckej konferencie
"Analytické metódy a zdravie loveka", ISBN 978-80-969435-7-9
- 128 -
CD 2
S 3
S 2
S 1
LE
CT
a
C
R
TV
b
WS
c
LE
d
hotel Falkensteiner, Bratislava
11. - 14. 10. 2010