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SOME ASPECTS OF IMPLEMENTATION DNA MARKERS IN
ANIMAL BREEDING
Vitomir Vidović, Milanko Stupar, Dragomir Lukač, Ljuba Štrbac
SUMMARY
DNA-based typing will replace blood group and protein marker typing, although
the adoption of a new system will require breeding animals that previously had been
typed to be re-tested using DNA markers. Commonly used types of genetic markers are:
RFLP (restriction fragment length polymorphism), SSLP (simple sequence length
polymorphism), AFLP (amplified fragment length polymorphism), RAPD (random
amplification of polymorphic DNA), VNTR (variable number tandem repeats),
microsatellite polymorphism, SNP (single nucleotide polymorphism), STR (short
tandem repeat), and MS (minisatellites). Aim of this paper is to review some of
available DNA markers in horse, sheep, pig and cattle breeding. The current
commercially available panel comprises 20 microsatellite horse markers: AHT4, AHT5,
ASB2, HMS3, HMS6, HMS7, HTG4, HTG10, VHL20, ASB17, ASB23, LEX33,
LEX3, CA425, HMS1, HMS2, HTG6, HTG7, UM011, AME. In sheep is known 528
markers, in pig 1446 and in cattle 3907 markers. Application of DNA-based methods in
animal breeding programs offer several advantages over conventional parentage assays
in terms of their accuracy and specificity, the ability to use a range of easily obtainable
sample tissues including hair, and laboratory analysis with commonly available
molecular biological reagents.
Key words: DNA markers, animal breeding
Primljeno: 27.08.2012
Prihvaćen: 25.10.2012
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