ÇUKUROVA ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ ...
ÇUKUROVA ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ ...
ÇUKUROVA ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ ...
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1.2.1.1.(1). Cu/Zn SOD ................................................................................ 17<br />
1.2.1.1.(2). Mn SOD ..................................................................................... 18<br />
1.2.1.1.(3). Fe SOD ....................................................................................... 19<br />
1.2.1.2. Katalaz (Hidrojen peroksit:hidrojen peroksit oksidoredüktaz)<br />
(CAT) (EC: 1.11.1.6) ........................................................................ 20<br />
1.2.1.3. Glutatyon Peroksidaz (glutatyon:hydrojen peroksit<br />
oksidoredüktaz) (GSH-Px) (EC: 1.11.1.9) ........................................ 21<br />
1.2.1.3.(1). Glutatyon redüktaz (glutatyon:NADP +<br />
oksidoredüktaz) (GSH-Rd) (EC: 1.8.1.7) .................................. 23<br />
1.2.1.3.(2). Glutatyon–S-Transferaz (RX:glutatyon R-transferaz)<br />
(GST) (EC: 2.5.1.18) ................................................................ 23<br />
1.2.2. Enzimatik Olmayan Antioksidanlar ........................................................ 23<br />
1.2.2.1. Glutatyon (GSH) ................................................................................ 23<br />
1.2.2.2. Vitamin E (Tokoferol) ....................................................................... 24<br />
1.2.2.3. Vitamin C (Askorbik asit) .................................................................. 25<br />
1.2.2.4. Vitamin A ........................................................................................... 26<br />
1.2.2.5. Melatonin ........................................................................................... 26<br />
1.2.2.6. Biluribin ............................................................................................. 26<br />
1.2.2.7. Albumin ............................................................................................. 27<br />
1.2.2.8. Ürik asit .............................................................................................. 27<br />
1.3.Pestisitler ........................................................................................................ 27<br />
1.3.1. İnsektisitler .............................................................................................. 27<br />
1.3.1.1. Diazinon (C12H21N2O3PS) ................................................................. 29<br />
2. ÖNCEKİ ÇALIŞMALAR .................................................................................. 31<br />
3. MATERYAL ve METOD ................................................................................. 35<br />
3.1. Materyal ........................................................................................................ 35<br />
3.1.1. Deney Böceklerinin Elde Edilmesi ......................................................... 35<br />
3.1.2. Deney Besinlerinin Hazırlanması ............................................................ 35<br />
3.1.2.1. Kontrol Besinin Hazırlanması ............................................................ 35<br />
3.1.2.2. Diazinon İçeren Besinlerin Hazırlanması .......................................... 38<br />
3.1.3. Enzim Aktivitelerinin Belirlenmesinde Kullanılan<br />
VIII