ADKG2008 - Namık Kemal Üniversitesi

Đlbağı,H., Rabenstein, F., Habekuss, A., Ordon, F., Çıtır, A., Cebeci, O. andBudak, H. 2008. Molecular, serological and aphid transmission studies ofBarley yellow dwarf virus-PAV and Cereal yellow dwarf virus-RPV in CanarySeed (Phalaris canariensis L.). Cereal Research Communications 36: 225-234. (Assoc. Prof.Dr. Đlbağı, H. and Prof.Dr. A. Citir. Namık Kemal University, Faculty of Agriculture,Department of Plant Protection, Tekirdağ-Turkey.) Abstract: Canary seed (Phalaris canariensis L.) is acereal crop belonging to the tribe Phalarideae of the Poaceae family. A prevailing virus infection, which causesdwarfing of plants and yellowing of leaves, was observed in canary seed fields in the Tekirdag province ofTurkey. The aim of this study was to identify, clone and sequence the cereal viruses naturally occurring on P.canariensis by employing serological tests as DAS-ELISA and TAS-ELISA tests combined with transmissionelectron microcopy (TEM) and molecular analysis. One hundred and one plant samples showing symptomswere collected and tested serologically using polyclonal antisera against Barley yellow dwarf virus-PAV(BYDV-PAV) and Cereal yellow dwarf virus-RPV (CYDV-RPV). The results of both immunoassays showedthat 48% of the samples were infected with BYDV-PAV, 2% with CYDV-RPV and 14% were mixed infection.36% of the samples were uninfected or infected at level below detection. Aphid transmission experimentsrevealed that barley (cv. Rubina) exhibited characteristic of CYDV-RPV. Investigations of infected canarygrass seedlings using transmission electron microscopy (TEM) supported the findings of serological tests andrevealed the presence of isometric particles of approximately 25 nm in diameter. These results were alsoconfirmed by using BYDV-PAV and CYDV-RPV specific primers. Sequence analysis of cDNA and probabletranslation products revealed a high level of homology to BYDV-PAV and CYDV-RPV isolates found in otherplant species. The sequence data obtained from this research were deposited in the EMBL/GenBank DataLibraries under the accession nos. EGO19056 and EF372272.Covelli, L., Kozlakidis, Z., Di Serio, F., Citir, A., Acikgoz, S., Hernandez, C.,Ragozzino, A., Coutts, R. H. A., Flores, R. 2008. Sequences of the smallestdouble-stranded RNAs associated with cherry chlorotic rusty spot andAmasya cherry diseases. Archives of Virology 153 (4): 759-762. (Professor A.Citir. Namık Kemal University, Faculty of Agriculture, Department of Plant Protection) Abstract: Cherrychlorotic rusty spot (CCRS) and Amasya cherry disease (ACD) are two disorders that affect cherry in Italy andTurkey. Both diseases have similar symptoms and are associated with a complex pattern of double-strandedRNAs (dsRNAs), absent from healthy-looking material. The dsRNAs were presumed to have a viral origin, andtheir characterization showed that: four comprise the genomic components of a member of a new tentativespecies of the genus Chrysovirus, two comprise the genomic components of a member of a new tentativespecies of the genus Partitivirus, and another four display their highest similarity with members of the familyTotiviridae. Because members of these three taxa typically infect fungi, and because microscopy observationshave identified a fungus-like organism in the affected leaf tissues, these data support a fungal etiology forCCRS and ACD diseases. In addition to the ten large dsRNAs, PAGE analysis of CCRS-affected materialrevealed at least another two bands in the lower part of the gels. We presumed that they were generated bysmall dsRNAs, given that the bands were detected in preparations enriched in dsRNAs. Here we report theirsequences.Kıvan, M., 2008. Development rate and lower temperature threshold in the eggs ofEurygaster integriceps (Heteroptera : Scutelleridae), Insect Scıence 15(2):163-166. (Prof.Dr. Kivan, M. Namık Kemal University, Faculty of Agriculture, Department of PlantProtection. Tekirdag-Turkey) Abstract: The sunn pest, Eurygaster integriceps Put. has a wide distribution inthe Palearctic region. It is the most important pest problem of wheat in Turkey. The objective of this study wasto attain better knowledge of the development of the sunn pest eggs. The lower temperature threshold anddevelopment rate of eggs were determined at 17, 20, 23, 26 and 32 degrees C +/- 1 degrees C in thelaboratory. A linear model was used to describe the developmental rate and temperature. The eggdevelopment required 90.9 degree-days above the theoretical threshold of 11.7 degrees C. The developmenttime was 17.6 +/- 0.1 days at 17 degrees C, and 4.5 +/- 0.01 days at 32 degrees C. Incubation time wasinversely related to temperature. The study showed that the eggs of E. integriceps needed shorter periods oftime to complete their development than immature stages of their parasitoids Trissolcus spp.Mirik, M., Selcuk, F., Aysan, Y., and Sahin, F. 2008. First outbreak of bacterialblack rot on cabbage, broccoli and brussels sprouts caused by Xanthomonascampestris pv campestris in the Mediterranean Region of Turkey. PlantDisease 92 (1): 176. (Diseses Not)33