2 - Akademos - Academia de Ştiinţe a Moldovei

AkademosObtained ResultsThe methodology and strategy of our investigationsallow using of Spirulina biomass for obtainingof two sanogenic products for apiculture – feed additiveApispir and BioR Sp preparation (Fig.1).Temperature:30-35 o C;pH 8-10;Ilumination:3-4 thous.lxaqua-etanolic extractwith bioactiveprinciples contentBioR SpSpirulina platensis CNM-CB-02Nutritive medium SP-1 preparationSpirulina cultivationBiomass separation by filtrationPurificationBiomassThe 1-st extractionand centrifugationResiduum IThe secondextraction andcentrifugationExtract II withbioactive principlesApispirPurificationChemicalcompounds ofbioelementsResiduumIIOthertehnologiesFig. 1. Integrate technologic scheme of BioR Sppreparation and feed additive Apispir obtaining.BioR Sp preparation contains substances withlow molecular mass such as: free amino acids andoligopeptides (up to 10kDa), carotenoids, pheophytin,intermediary compounds of carbohydrates andlipids metabolism, macro- and microelements withantioxidant action. The preparation demonstratesthe antioxidant properties and the capacity to stabilizethe cellular and lissome membranes by the normalizationof glutathione metabolism. The presenceof amino acids and oligopeptides, as well as suchmicroelements as Mn, Fe, Zn, Cu, Se, Cr stimulatesthe process of regeneration of tissues and has a positiveeffect on cellular and humoral immunity.APISPIR contains proteins, carbohydrates (includingsulfated polysaccharides), and microelements.The action mechanism of this product canbe attributed to the properties of the constituentsubstances. The proteic composition offers the totalessential amino acids content with a high efficiency(up to 87%) and utilization (92%). There are thefollowing amino acids with immunostimulative andantioxidant action): aspartic and glutamic acids, alanine,cysteine, glycine and serine, threonine, thriptophanand valine.The utilization of BioR Sp preparation during autumnnutrition for honey reserves supplementing forbees wintering, provides an activation of physiologicaland biochemical processes in the organisms ofworking bees (Tab.1).Table 1. Dynamics of some biochemical indices onbees after BioR Sp autumn administration (in %).VariantBeforesupplem.Control (puresyrup)Biochemical indicesALT AST LDH Al. Ph A. Ph T.Pr.100,0 100,0 100,0 100,0 100,0 100,097,8 100,0 102,1 71,6 23,2 77,5BioR Sp 126,1 200,0 174,0 93,1 57,1 102,6ALT – alaninaminotransferase (mmol/h.l); AST –aspartataminotranspherase (mmol/h.l); LDH – lactatdehydrogenase(mmol/s.l); Al. Ph. – alkaline phosphatase(nmol/s.l); A. Ph. – acide phosphatase (nmol/s.l);T.Pr. – total proteins.The active components of preparation increasedthe physiologic capacities of working bees. Thebees’ families were in good condition after wintering,without any signs of diarrhea at bees or nutritionoxidation and fermentation.The utilization of Apispir as feed additive forspring nutrition has stimulated the cowering spawnof queen, increasing of new generation growth andthe quantity of collected honey. The remedy wasconsumed by the bees, increasing up to 18-29%the number of young generation, the honey quantity,collected after acacia pollination, up to 22-53%(Fig.2).160140120100806040200100%Control129%153%ApispirCowering spawnHoneyFig. 2. The cowering spawn and honey quantityobtained after APISPIR in spring administration.In conclusion, APISPIR and BioR Sp represent thenew sanogenic, ecological products, without adverseeffects with high efficacy that could be used inlower quantities due to the production technologyand bioactive components. The remedies can beused in the apiculture for stimulation of bees families’growing, increase of apiculture products quantity,enhance of bees immune status, and CCD (ColonyCollapse Disorder) eradication.82 - nr. 4(11), decembrie 2008