rezumatele tezelor de doctorat susținute în anii 2009 – 2010

UNIUNEAEUROPEANĂGUVERNUL ROMÂNIEIMINISTERUL MUNCII, FAMILIEI ŞIPROTECȚIEI SOCIALEAMPOSDRUFondul Social EuropeanPOS DRU2007-2013Instrumente Structurale2007-2013Investeşte în oameni!FONDUL SOCIAL EUROPEANProgramul Operaţional Sectorial pentru Dezvoltarea Resurselor Umane 2007 – 2013OIPOSDRUUNIVERSITATEADE VEST DINTIMIŞOARASummaryStudy of enzymatic reaction was carried out according to the following approaches:Enzyme kinetics - a useful tool for studying properties of enzymes.Using curves instead total kinetic analysis of initial reaction rates to determine kinetic constants ofenzymes have some advantages.Initial velocity method, widely used, is cumbersome and requires a large number of experiments.Using enzymes as diagnostic tool in clinical laboratoriesThe original contributions.Making a documented literature on the enzyme (structure and classification) and the methodologyfor measuring the activity in blood, specifying their main correlations with myocardial infarction.Documentation relating to optimize systems and its use to obtain better performance of themethods of investigation.Choice of measurement method and the methodology of work (dry or wet chemistry) used in theexperimental partInternal quality control methodologyResults. The studied cases. Distribution of myocardial infarction by sex and age groups in ourpatients followed between 2004 to 2009.Dividing groups of patients with myocardial infarction diagnosed and confirmed by sex and agegroup we found the following percentages- 27% of men aged 55 years- 36% of men aged over 55 years- 9% of women aged 55 years- 28% of women aged over 55 yearsGraphs corresponding correlations between ALT, AST, CK, CK-MB and LDH and mathematicalmodels and experimental, not separately from other laboratory tests clearly documents relating to theinstallation myocardial infarction.Finally in the fourth and last part of the work I have followed developments of troponins serum valuesI and T (cTnI and cTnT) in patients who were on renal dialysis program. Cardiac disease is theleading cause of morbidity and mortality in patients with end-stage renal disease (ESRD). Myocardialischaemia in dialysis patients may be asymptomatic, and may occur in the absence of criticalstenoses of coronary arteries. Patients with end stage renal failure undergoing chronic hemodialysishave a high incidence of cardiac events and of false-positive increases in myoglobin as well ascreatine kinase and its MB isoenzymes. Many investigators hypothesize that uremic induced skeletalmyopathy may be responsible for increased troponins in renal failure because uremia may promotere-expression of cardiac TnT from injured or regenerating skeletal muscle fibers.The objective of this study was to investigate the correlation between increasing cTnI, cTnT andcardiac structural and functional abnormalities in a group of patients with ESRD.303POSDRU 21/1.5/G/38347”Parteneriat local pentru ridicarea nivelului şcolilor doctorale şi valorificarea potenţialului uman din cercetare îndomenii prioritare ale etapei postaderare a României la UE”