Departamentul de Biochimie şi Biologie Moleculară - dorinţa de a construiLucrări publicate în reviste cotate ISI,în volumele unor conferinţe internaţionalePapers Published in ISI (ranked) Journals andin Proceedings of International Conferences Paraschiv S, Otelea D, Baicus C,Tinischi M, CostacheM, Nucleoside reverse transcriptase inhibitor resistancemutations in subtype F1 strains isolated from heavily treatedadolescents in Romania, Int J Infect Dis.,13(1), 81-89, 2009 Huculeci R, Dinu D, Staicu C, Munteanu C, CostacheM, Dinischiotu A, Malathion- Induced Alteration of theAntioxidant Defence System in Kidney, Gill and Intestineof Carassius auratus gibelio, Environ Toxicol, 24(6),523-530, 2009 Ciubǎr R, Ciofrangeanu C, Gǎlǎţeanu B, Manea S,CîmpeanA, Iordǎchescu D, Biochemical investigation of someproteins from human primary teeth to evaluate heavymetal pollution, Key Eng Mat, 415, 53-56, 2009. Vatasescu R, Predoi D, Ungureanu F, Costache M,Study of iron oxide nanoparticles coated with dextrinobtained by coprecipitation JOAM, 10(3):693-696,2008 Predoi D, Pasuk I, Trusca R, Costache M, Calciumphosphate ceramics for biomedical applications, JOAM,10(8):2151-2155 2008 Scorei R, Ciubar R, Ciofrangeanu C, Mitran V,Cîmpean A,Iordǎchescu D, Comparative effects of boricacid and calcium fructoborate on breast cancer cells,BiolTrace Elem Res, 122,197-205, 2008. Dinu D, Nechifor M T, Costache M, Dinischiotu A,Enzymes with new biochemical properties in the pectinolyticcomplex produced by Aspergillus niger MIUG 16,J Biotech 131(2):128-137, 2007 Simion M, Kleps I, Neghina T, Angelescu A, Miu M,Bragaru A, Danila M, Costache M, Nanoporous silicon matrixused as biomaterial, J Alloys Comp, 434830-832, 2007 Predoi D, Barsan M, Andronescu E, Costache M,Hydroxyapatite-iron oxide bioceramic prepared usingnano-size powders, JOAM, 9(11):3609-3613 2007 Predoi D, Ghita RV, Ungureanu F, Negrila C, CostacheM, Characteristics of hydroxyapatite thin films, JOAM,9(12):3827-3831 2007 Paraschiv S, Munteanu M, Staicu C, Dinu D, CostacheM, Dinischiotu A, Biochemical response in intestine andgills of Carassius auratus gibelio to acute manganese(II)intoxication, Rev Roum Chim, 51(12):1175-1179 2006 Fernandez P M, Costache M, Oriol R, Mollicone R,Point mutations and deletion responsible for the BombayH null blood groups, Vox sang, 663, 1-10,1998 Dojană N, Costache M, Dinischiotu A, The Activity ofsome digestive enzymes in domestic rabbits before andafter weaning, AnimSci, 66, 501-517,1998 Costache M et all, Advances in molecular genetics ofα-2- and α-3/4-fucosyltransférases, Transfus Clin Biol, 4,367-382,1997 Costache M et all, Evolution of fucosyltransferasesgenes in vertebrates, JBC, 272, 29721-29728,1997 Oulmouden, Costache M, Mollicone R, Oriol R, Mole -cular cloning and expression of a bovine α(1,3)- Fucosyltransferasegene homologous to a putative ancestor geneof the human FUT3-FUT5-FUT6 Cluster, JBC, 272, 13,8764-8773,1997 Elmgren A, Mollicone R, Costache M, Oriol R, LarsonG., Significance of individual point mutations, T202C andC314T, in the human Lewis(FUT3) gene for expressionof the Lewis antigens by the human α(1,3/1,4)fucosyltransferase,Fuc-TIII, JBC, 272,21994-21998,1997colegii la specializare la cele mai bune universităţi dinEuropa dar şi datorita determinarii celor care s-au întors laUniversitatea din Bucureşti, cu credinţa că vor şi pot implementaacasă concepte moderne în educaţie şi cercetareşi pot construi laboratoare moderne pentru formarea profesionalăa studenţilor şi absolvenţilor secţiei de Biochimie. Înacest context, la declanşarea programului CNCSIS pentrufinanţarea învăţământului superior, colectivul condus dedoamna Profesor Doctor Dana Iordăchescu a avut posibilitateaaccesării primelor fonduri. Proiectele CNFIS “Laboratorde culturi celulare animale” 1999 –2002, “Programcomplex de studii aprofundate şi doctorat în domeniul bio -logiei moleculare” 1998-2002, şi BCUM B12 “Centru decercetare şi formare în domeniul biologiei moleculare”, coordonat de Doamna Profesor Marieta Costache, au pusbazele infrastructurii moderne de cercetare a Departamentulde Biochimie şi Biologie Moleculară.Dorinţa colectivului de a realiza un salt calitativ încerce tarea de profil in România s-a continuat prin accesareade proiecte naţionale (CNCSIS, PNCDI, CEEX, PNII).Valoa rea totală a investiţiilor în infrastructură este de peste8.890.000 lei (1998-2009) din care 7.000.000 lei în 2005-2009.Cele 4 laboratoare de biologie moleculară sunt acreditateISO 17025/2005 şi dotate cu echipamente performantepentru cercetare, consultanţă şi sevicii în domeniul Genomicii,Transcriptomicii şi Proteomicii fiind dotate cu ana lizoaregenetice, aparate PCR şi platforme Real-Time PCRşi microarray. Laboratoarele de biologie celulară, culturi şimicroscopie au hote cu dublu flux laminar, incubatoareCO 2 , microscoape optice, flow-citometru, criotom, microtom,microscoape directe şi inversate cu fluorescenţă, concializein the best universities in Europe, but also the determinationof those who returned to the University ofBucharest, together with the belief that they can implementmodern concepts education and research at home and canbuild modern laboratories for the professional training ofstudents and graduates of the Biochemistry department haveproven instrumental. In this context, at the onset of theCNCSIS program to finance higher education, the teamheaded by PhD Dana Iordachescu was able to access the firstfunds. Projects such as CNFIS "Laboratory of animal cellcultures" 1999-2002, "Comprehensive program of postgraduateand doctorate studies in molecular biology" 1998-2002, and BCUM-BM "Research and training center inmolecular biology", coordinated by Prof. PhD MarietaCostache, founded modern research infrastructures of theDepartment of Biochemistry and Molecular Biology.The desire of the research team to achieve a qualitativeleap in the research field in institutions from Romania led totheir striving to access further national projects (CNCSIS,PNCDI, CEEX PNII). The total value of investment in infrastructureis worth more than 8,890,000 lei (1998-2009)of which 7,000,000 lei between 2005-2009.The 4 molecular biology laboratories are ISO17025/2005 accredited and fitted with high-performanceequipment for undertaking research, consultancy and servi -ces in the field of Genomics, Proteomics and Transcriptomics,the infrastructure including genetic analyzers, PCRequipments and Real-Time PCR and microarray platforms.Cell biology, cultures and microscopy laboratories have duallaminar flow hoods, CO2 incubators, microscopes, opticalflow cytometers, cryotome, microtome, direct and invertedfluorescence microscopes, confocal, laser microdissection.In the 3 biochemistry laboratories we undertake qualitative22
Laborator BiochimieBiochemistry LaboratoryLaborator microscopieMicroscopy LaboratoryLaborator electroforezăElectrophoresis LaboratoryLaborator secvențiereSequencing Laboratoryfocal, microdisecţie laser. In cele 3 laboratoare de biochimiese realizează analize calitative şi cantitative specializate cuspectrofotometre UV/VIS si sisteme de electroforeză, blot,video/foto documentare, FPLC şi HPLC, toate de ultimăgeneraţie.Activităţile de cercetare orientate către analiza genomicăşi epigenomică (izolare şi purificare, clonare moleculară,amplificare in vitro, secvenţiere, amprentare genică, metilare,analiză SNPs prin Real-Time şi ADN Microarray) suntinterconectate cu cele de diagnostic molecular (LOH, MSI,MLPA).Dotările performate şi specializarea personalului au permisorientarea către analiza transcriptomică şi proteomicăprin îmbinarea tehnicilor moderne (separare şi purificareARN, Real Time RT-PCR, transcripţie şi traducere in vitro,ARN interferenţă, ARN microarray, HPLC, FPLC) cu experienţacolectivului către tehnicile clasice (purificare proteineşi peptide, cinetică enzimatică, electoforeză mono/bidimensională).Resursa umană implicatăÎn cadrul Departamentului de Biochimie şi BiologieMoleculara se desfăşoară activităţile de cercetare aferenteproiectelor câstigate prin competiţii naţionale şi internaţionale,studiile experimentale din cadrul programelor delicenţă, master şi pregătire doctorală. Componenta decerce tare este structurată pe 6 direcţii de cercetare (Biodiandquantitative specialized analyses with UV/VIS Spectophotometers,electrophoresis, blot, video/photo documentation,FPLC and HPLC systems, all belonging to the latestgeneration.Research activities oriented towards epigenomic and genomicanalysis (isolation and purification, molecularcloning, in vitro amplification, sequencing, gene fingerprinting,methylation, SNPs analysis by DNA Microarrayand Real-Time) are interconnected with the molecular diagnosis(LOH, MSI, MLPA)High-performance facilities and staff specializationallowed the orientation towards transcriptomics and proteomicsby combining modern techniques (RNA separationand purification, Real Time RT-PCR, in vitro transcriptionand translation, RNA interference, RNA microarray, HPLC,FPLC) with the collective experience of the research team withclassical approaches (proteins and peptides purification,enzyme kinetics, single/two-dimensional electrophoresis).Human ResourcesThe research activities of the Department of Bioche -mistry and Molecular Biology are related to the projects obtainedin national and international competitions,expe rimental studies of undergraduate, master's programsand PhD training. The research compartment has 6 directions(genetic biodiversity, molecular phylogeny, tissue engineering,biomaterials biocompatibility, molecularcharacterization of different human diseases, molecularDepartment of Biochemistry and Molecular Biology - desire to build23
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