Clujul Medical - Iuliu Haţieganu

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Clujul Medical 2006 vol. LXXX - nr. 1 Intens. x104 8 6 4 2 0 3. +MS2(194.9), 2.6-3.0min 194.9 175 180 185 190 195 m/z Fig. 3. Electrospray positive ion MS spectra for caffeine. The caffeine content in all samples was determined on the basis of the calibration curve obtained by applying the proposed method. The standard curve was prepared over 7 different concentrations in the range 0.26-26 µg/ml for caffeine. Peak areas were plotted against the corresponding concentrations using a quadratic model with a 1/Y weighting scheme in order to generate the calibration curve (determination coefficients are greater than 0.995). Fig. 4. The calibration curve for caffeine. The developed HPLC/MS method was then used for quantification of caffeine in 7 instant coffee samples and 11 cappuccino samples that are frequently consumed by Romanian people. The results in Table I show that the concentration of caffeine in the instant coffee samples is between 3.277 and 4.753 g%, while in the cappuccino samples the content in caffeine is much smaller, between 0.013 and 0.605 g%. These results are in accordance with the dose of caffeine estimated for each beverage based on the usual volume dispensed, which is of 65-100 mg caffeine per serving of instant coffee and of 0,002-0,080 g caffeine per serving of cappuccino 4 . 192
Clujul Medical 2006 vol. LXXX - nr. 1 Table I. Caffeine concentration in instant coffee and cappuccino samples Product name Producer Caffeine g% Instant coffee 1 Amigo-soluvel Amigo- Brazil 3.376 2 Elita instant coffee red Elite Industries Israel 4.753 3 Elita instant coffee green Elite Industries Israel 4.276 4 Mocca-gold sol.-Alka Alka-Romania 3.731 5 Nescafe Brasero Nestle France 4.620 6 Nescafe Classic Nestle Brazil 3.634 7 Davidoff-instant coffee Tchibo GmbH Germany 3.277 8 Jacobs 3 in 1 Supreme Imex- Bucuresti 0.470 9 La Festa 3 in 1 Maspex Polonia 0.356 10 La Festa 3 in 1 Latte Maspex Polonia 0.078 11 La Festa 3 in 1 Mocca Maspex Polonia 0.372 Cappuccino 1 Jacobs cappuccino classic Supreme Imex- Bucuresti 0.364 2 Elite cappuccino classic Elite Romania-Bucuresti 0.490 3 Elite cappuccino vanilla Elite Romania-Bucuresti 0.605 4 Elite cappuccino peanuts Elite Romania-Bucuresti 0.406 5 La Festa cappuccino.orig.clasic Maspex Polonia 0.016 6 La Festa cappuccino Jamaica rum Maspex Polonia 0.017 7 La Festa cappuccino Royal nutto Maspex Polonia 0.013 Conclusions A rapid and simple LC/MS method was elaborated, that proved to be suitable for quantification of caffeine in different brands of instant coffee and cappuccino. The developed method can be successfully used for fast and accurate determinations of caffeine in coffee, especially because of the performance of detection technique, which provides a very short time of analyze. References 1. GALASKO GTF, FURMAN KI, ALBERTS E: The caffeine content of nonalcoholic beverages, Food Chem.Toxicol., 1989: (27), 49 – 51. 2. POPA D.S. : Curs de toxicologie pentru studenţii colegiului de tehnicieni de farmacie ,Ed. Medicala Universitara “Iuliu Hatieganu”, Cluj-Napoca, 2004, 118- 120. 3. NAKASHIMA K, INOUE K, MAYAHARA K et al.:Use of 3-(1,8naphthalimido)propyl-modified silyl silica gel as a stationary phase for the highperformance liquid chromatographic separation of purine derivatives, J.Chromatogr.A, 1996: 107 - 113. 4. GOLDBERGER BA, LESSIG MC, MCCUSKER RR, CONE EJ, GOLD MS: Evaluation of Current Caffeine Content of Coffee Beverages: Recommendations for Clinicians Regarding Caffeine Exposure, Biol. Psychiatry 53, 2003: 570. 193
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Clujul Medical Revistă de Medicin
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Articole de orientare Clujul Medica
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VOICHITA SĂBĂDUŞ, D. BORZEA, DIA
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CAMELIA GEORGETA RĂCĂREANU, GABRI
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Articole de orientare ROLUL NEUROKI
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DETECŢIA REARANJAMENTELOR SUBTELOM
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