Biological_Microscope

How To lTack a Mlcroscoplc Image
Dlrectlon of moving the Image
©The image observed through the microscope moves in
directions opposite to the actual up-down and left-right
movements of the specimen.
Total Magnlflcatlon
The size of the specimen image for observation i§ obtained by multiplying the eyepiece magnlflcation by the
objective magnificatic)n. This value is referred to as total magnificatictn.
Example: Eyepiece (lox) x objective (40X) = 400X
Fle8olutlon
Plesolu[lc)n delerrTilnes how nnely a lens ls able to distinguish the delail5 ctf a sF)ecimeri
The re§olutlon of a mlcrc)scope 1§ malnly determined by the ablllty o[ the objeclive and is scarcely related [o that of the
eyepiece8. The only functic)n of the eyepieces ls to magnlfy an Image already re§ctlved by the cib|ective
Althouoh hath eonflguratlon§ above provide the same total maonlflcation, the higher maonifi[atlon of THE c)bjective
yields a better speclmen reso[utlon
Fleld Number (FN)
The field number ls the dlameter of the image cibserved through an eyepiece, represented in mnlimeters,
10Xeyepiece.18mm
Actual Fleld of Vlow
WHC15X.12mm
£88[#Lha°|i9oTamc'tual
field of vlew.
The actual field of view is the size on the specimen actually
observed in the microscope. This yields approximate size of
the actual specimen.
Actual field of view - Pleld number/Objective magnification
When the lox eyepiece above and lox objective are used,
the actual field of view is equal to:
Actual field of view -18/10 -1.8 mm
Actual field of
view: 1.8 mm