2002 - Instituto de QuÃmica - USP
2002 - Instituto de QuÃmica - USP
2002 - Instituto de QuÃmica - USP
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Ohara AugustoLABORATÓRIO DE BIOQUÍMICA DERADICAIS LIVRES E EPRAo longo dos anos, o nosso grupo <strong>de</strong> pesquisatem contribuído para a compreensão da Bioquímica<strong>de</strong> radicais livres e oxidantes explorando, inclusive,as potencialida<strong>de</strong>s da espectroscopia <strong>de</strong> EPR(ressonância paramagnética eletrônica) para <strong>de</strong>tectarradicais livres em sistemas biológicos, células e animaisexperimentais. Os nossos estudos recentescontribuiram para colocar em foco espécies que erampraticamente ignoradas em Biologia até 1990 comoo peroxinitrito, dióxido <strong>de</strong> nitrogênio e anion radicalcarbonato. Presentemente estudamos os papéis<strong>de</strong>ssas espécies em mecanismos microbicidas e emprocessos inflamatórios.SummaryOur research group has contributed to the un<strong>de</strong>rstandingof the Biochemistry of free radicals and oxidantsexploring, among other methodologies, thepotentialities of the EPR specroscopy (electron paramagneticresonance) to <strong>de</strong>tect free radicals in biologicalsystems, cells and whole animals. Our recentstudies have contributed to bring into focus speciesthat were practically ignored in Biology up to the90’s such as peroxynitrite, nitrogen dioxi<strong>de</strong> and thecarbonate radical anion. Presently, we are examiningthe role of these species in microbicidal mechanismsand in inflammatory processes.Publicações:Augusto, O., De Menezes, S. L., Linares, E., Romero, N., Radi, R. andDenicola, A. (<strong>2002</strong>) EPR <strong>de</strong>tection of glutathyl and hemoglobin-cysteinylradicals during the interaction of peroxynitrite with human erythrocytes.Biochemistry, in press.Nakao, L. S., Fonseca, E. and Augusto, O. (<strong>2002</strong>) Detection of C8-(1-hydroxyethyl)guanine in liver RNA and DNA from control and ethanoltreatedrats. Chem. Res. Toxicol. 15, 1248-1253.Augusto, O. Bonini, M. G., Amanso, A. M., Linares, E., Santos, C. C. X.and <strong>de</strong> Menezes, S. L. (<strong>2002</strong>) Nitrogen dioxi<strong>de</strong> and carbonate radicalanion: two emerging radicals in Biology. Free Radic. Biol. Med. 32, 841-859.Bonini, M. G., Mason, R. P. and Augusto, O. (<strong>2002</strong>) The mechanism bywhich 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (tempol) divertsperoxynitrite <strong>de</strong>composition from nitrating to nitrosating species. Chem.Res. Toxicol. 15, 506-511.Menezes, S. L., and Augusto, O. (2001) EPR <strong>de</strong>tection of glutathionyland protein-tyrosyl radiclas during the interaction of peroxynitrite withmacrophages (J774). J. Biol. Chem. 276, 39879-39884.Linares, E., Giorgio, S., Mortara, R. A., Santos, C. X. C., Yamada, A. T.and Augusto, O. (2001) Role of peroxynitrite in macrophage microbicidalmechanisms in vivo revealed by protein nitration and hydroxylation.Free Radic. Biol. Med. 30, 1234-1242.Bonini, M. G. and Augusto, O. (2001) Carbon dioxi<strong>de</strong> stimulates theproduction of thiyl, sulfinyl, and dissulfi<strong>de</strong> radical anion from thiol oxidationby peroxynitrite. J. Biol. Chem. 276, 9749-9754.Departamento <strong>de</strong> Química Bioquímica Fundamental77<strong>Instituto</strong> <strong>de</strong> Química - Pesquisa 2001 - <strong>2002</strong>