2002 - Instituto de Química - USP

Ohara AugustoLABORATÓRIO DE BIOQUÍMICA DERADICAIS LIVRES E EPRAo longo dos anos, o nosso grupo de pesquisatem contribuído para a compreensão da Bioquímicade radicais livres e oxidantes explorando, inclusive,as potencialidades da espectroscopia de EPR(ressonância paramagnética eletrônica) para detectarradicais livres em sistemas biológicos, células e animaisexperimentais. Os nossos estudos recentescontribuiram para colocar em foco espécies que erampraticamente ignoradas em Biologia até 1990 comoo peroxinitrito, dióxido de nitrogênio e anion radicalcarbonato. Presentemente estudamos os papéisdessas espécies em mecanismos microbicidas e emprocessos inflamatórios.SummaryOur research group has contributed to the understandingof the Biochemistry of free radicals and oxidantsexploring, among other methodologies, thepotentialities of the EPR specroscopy (electron paramagneticresonance) to detect free radicals in biologicalsystems, cells and whole animals. Our recentstudies have contributed to bring into focus speciesthat were practically ignored in Biology up to the90’s such as peroxynitrite, nitrogen dioxide and thecarbonate radical anion. Presently, we are examiningthe role of these species in microbicidal mechanismsand in inflammatory processes.Publicações:Augusto, O., De Menezes, S. L., Linares, E., Romero, N., Radi, R. andDenicola, A. (2002) EPR detection of glutathyl and hemoglobin-cysteinylradicals during the interaction of peroxynitrite with human erythrocytes.Biochemistry, in press.Nakao, L. S., Fonseca, E. and Augusto, O. (2002) Detection of C8-(1-hydroxyethyl)guanine in liver RNA and DNA from control and ethanoltreatedrats. Chem. Res. Toxicol. 15, 1248-1253.Augusto, O. Bonini, M. G., Amanso, A. M., Linares, E., Santos, C. C. X.and de Menezes, S. L. (2002) Nitrogen dioxide and carbonate radicalanion: two emerging radicals in Biology. Free Radic. Biol. Med. 32, 841-859.Bonini, M. G., Mason, R. P. and Augusto, O. (2002) The mechanism bywhich 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (tempol) divertsperoxynitrite decomposition from nitrating to nitrosating species. Chem.Res. Toxicol. 15, 506-511.Menezes, S. L., and Augusto, O. (2001) EPR detection of glutathionyland protein-tyrosyl radiclas during the interaction of peroxynitrite withmacrophages (J774). J. Biol. Chem. 276, 39879-39884.Linares, E., Giorgio, S., Mortara, R. A., Santos, C. X. C., Yamada, A. T.and Augusto, O. (2001) Role of peroxynitrite in macrophage microbicidalmechanisms in vivo revealed by protein nitration and hydroxylation.Free Radic. Biol. Med. 30, 1234-1242.Bonini, M. G. and Augusto, O. (2001) Carbon dioxide stimulates theproduction of thiyl, sulfinyl, and dissulfide radical anion from thiol oxidationby peroxynitrite. J. Biol. Chem. 276, 9749-9754.Departamento de Química Bioquímica Fundamental77Instituto de Química - Pesquisa 2001 - 2002