2002 - Instituto de Química - USP

Walter Ribeiro TerraASPECTOS MOLECULARES,CELULARES E EVOLUTIVOS DADIGESTÃO NOS INSETOSO projeto visa detalhar a nível molecular osmodelos propostos para o processo digestivo dosinsetos, procurando descrever alvos para novosprocedimentos de controle. O estudo envolve acaracterização de enzimas digestivas selecionadas,incluindo sua estrutura 3D, sua expressão diferencialem várias condições nutricionais e seus mecanismosde secreção. As técnicas usadas são cromatográficas,DNA recombinante com bibliotecas subtrativas eproteômicas. Vários insetos-modelos são usados parainferências evolutivas.SummaryMolecular, cellular and evolutionary aspects ofinsect digestion. The group aims to understand inmolecular detail the digestive process in insects, lookingfor new targets for insect control. The study ofselected digestive enzymes includes their 3D structures,their differential expression at several nutritionalconditions, and their mechanisms of secretion.The experimental approach includes chromatography,the use of recombinant DNA technology withsubtractive libraries and proteomics. Several modelinsects are used to support evolutionary inferences.Publicações:(1) Brito L.O., Lopes, A.R., Parra, J.R.P., Terra, W.R., Silva-Filho, M.C. (2001)Adaptation of tobacco budworm Heliothis virescens to proteinase inhibitorsmay be mediated by the synthesis of new proteinases. Comp.Biochem. Physiol., 128B, 365-375. (2) Marana S.R., Jacobs-Lorena M.,Terra W.R., Ferreira C. (2001) Amino acid residues involved in substratebinding and catalysis in a insect digestive b-glicosidase. Biochim. Biophys.Acta. 1545, 41-52. (3) Cristofoletti P.T., Ribeiro A.F., Terra W.R. (2001)Apocrine secretion of amylase and exocytosis of trypsin along the midgutof Tenebrio molitor larvae. J. Insect Physiol. 47, 143-155. (4) SilvaC.P., Terra W.R., Lima R.M. (2001) Differences in midgut serine proteinasesfrom larvae of bruchid beetles Callosobruchus maculatus andZabrotes subfasciatus. Arch. Insect Biochem. Physiol. 47, 18-28. (5) SilvaC.P., Terra W.R., Xavier-Filho J., Grossi de Sá M.F., Isejima E.M., Damatta,R.A., Miguens, F.C., Bifano T.D. (2001) Digestion of legume starch granulesby larvae of Zabrotes subfasciatus (Coleoptera: Bruchidae) and theinduction of a-amylase in response to different diets. Insect Biochem.Molec. Biol. 31, 41-50. (6) Pompermayer P., Lopes A.R., Terra W.R., ParraJ.R.P., Falco M.C., Silva-Filho M.C. (2001) Effects of soybean proteinaseinhibitor on development, survival and reproductive potencial of the sugarcaneborer, Diatraea saccharalis. Ent. Exp. Appl. 99, 79-85. (7)Grossmann G.A., Terra W.R. (2001) a-Galactosidases from the larval midgutof Tenebrio molitor (Coleoptera) and Spodoptera frugiperda (Lepidoptera).Comp. Biochem. Physiol. 128B, 109-122. (8) Ferreira, A.H.P.,Marana S.R., Terra W.R., Ferreira, C. (2001). Purification, molecular cloning,and properties of a b-glicosidase isolated from midgut lumen ofTenebrio molitor (Coleoptera) larvae. Insect Biochem. Molec. Biol. 31,1065-1076. (9) Terra W.R. (2001) The origin and functions of the insectperitrophic membrane and peritrophic gel. Arch. Insect Biochem. Physiol.47, 47-61. (10) Bolognesi R., Ribeiro A.F., Terra W.R., Ferreira C. (2001)The peritrophic membrane of Spodoptera frugiperda: secretion ofperitrophic and role in immobilization and recycling digestive enzymes.Arch. Insect Biochem. Physiol. 47, 62-75. (11) Silva, C.P., Terra, W.R.,Grossi-de-Sá, M.F., Samuels, R.L., Isejima, E.M., Bifano, T.D., Almeida,J.S. (2001) Induction of digestive a-amylase in larvae of Zabrotessubfasciatus (Coleóptera: Bruchidae) in response to ingestion of comonbean a-amylase inhibitor 1. J. Insect Physiol., 47, 1283-1290. (12) Ferreira,A.H.P., Ribeiro, A.F., Terra, W.R., Ferreira, C. (2002) Secretion of b-glycosidaseby middle midgut cells and its recycling in the midgut of Tenebriomolitor larvar. J. insect Physiol., 48, 113-118. (13) Marana, S.R., Lopes,A.R., Juliano,L., Juliano, M.A., Ferreira, C., Terra, W.R. (2002) Subsites oftrypsin active site favor catalysis of substrate binding. Biochem. Biophys.Res. Comm., 290, 494-497. (14) Marana, S.R., Terra, W.R., Ferreira, C.(2002) The role of amino acid residues Q39 and E451 in the determinationof the specificity of an insect b-glycosidase. Eur. J. Biochem., 269,3705-3714. (15) Cristofoletti, P.T., Ribeiro, A.F., Deraison, C., Rahbe, Y.,Terra, W.R. (2002) Midgut adaptation and digestive enzyme distributionin a phloem feeding insect, the pea aphid. Acyrthosiphon pisum. J. InsectPhysiol., no prelo. (16) Azevedo, T.D., Terra, W.R. Ferreira, C. (2002)Purification and characterization of three b-glycosidases from midgut ofthe sugar borer, Diatraea saccharalis. Insect Biochem. Molec. Biol., noprelo. (17) Ferreira, A.H.P., Terra, W.R., Ferreira, C. (2002) Characterizationof a b-glycosidase highly active on disaccharides and of a b-galactosidasefrom Tenebrio molitor midgut lumen. Insect Biochem. Molec.Biol., no prelo. (18) Lopes, A.R., Terra, W.R. (2002) Purification, propertiesand substrate specificity of a digestive trypsin from Periplanetaamericana (Dictyoptera) adults. Submetido à publicação.Departamento de Química Bioquímica Fundamental107Instituto de Química - Pesquisa 2001 - 2002