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STRUCTURE OF GENETIC DIVERSITY AMONG COMMON BEAN
VARIETIES OF MIDDLE AMERICAN AND ANDEAN ORIGINS
USING NEW DEVELOPED MICROSSATELLITES
Luciana Lasry Benchimol ( 1 ), Tatiana de Campos ( 2 ) , Sérgio Augusto Morais Carbonell ( 3 ),
Alisson Fernando Chioratto ( 3 ), Eduardo Fernandes Formighieri ( 4 ) and Anete Pereira de Souza ( 5 )
ABSTRACT
A common bean genomic library was constructed for the ‘IAC-UNA’ variety enriched for (CT) and (GT) for
microsatellite motifs. From 1,209 sequenced clones, 714 showed microsatellites distributed over 471
simple and 243 compound motifs. GA/CT and GT/CA were the most frequent motifs found among these
sequences. A total of 123 microsatellites had already been characterized. From these, 93 were polymorphic
(75.6%), 27 monomorphic (22.0%), and 3 (2.4%) did not amplify at all, in evaluating 22 common bean
materials selected from Agronomic Institute Germplasm Bank. The number of alleles per locus varied
from 2 to 9, with an average of 2.89. The polymorphic information content (PIC) of each marker varied from
0.04 to 0.83, with 0.46 average value. Cluster and principal coordinate analysis were in agreement to
those originally assigned for Middle American and Andean gene pools. Low polymorphism levels detected
could be associated to domestication process. These microsatellites could then be a valuable resource
for the bean community because of their employment as new markers for genetic studies.
Key words: microsatellites, Simple Sequence Repeats (SSRs), Common Bean, Phaseolus vulgaris L,
SSR-Enriched Libraries, molecular markers.
( 1 ) Biologist, cientific researcher, “Centro de Pesquisa e Desenvolvimento de Recursos Genéticos” Vegetais (CPDRGV/IAC),
Caixa Postal 28, 13001-970, Campinas, SP. E-mail: llasry@iac.sp.gov.br;
( 2 ) Biologist, PhD student (Capes schoolarship), “Centro de Biologia Molecular e Engenharia Genética” (CBMEG), Campinas, SP.
E-mail: tatyuni@unicamp.br;
( 3 ) Agronomist, cientific researcher; “Centro de Análise e Pesquisa Tecnológica do Agronegócio dos Grãos e Fibras” (IAC). E-
mail: carbonel@iac.sp.gov.br; afchioratto@iac.sp.gov.br
( 4 ) Bioinformat, PhD student (CAPES schoolarship) of “Laboratório de Genômica e Expressão”, UNICAMP. E-mail:
eduformi@lge.ibi.unicamp.br
( 5 ) Agronomist, Profa. Dra. MS5, Bolsista do CNPq, CBMEG/DGE, IB.
Financial Support: FAPESP.e CNPq.
Documentos, IAC, Campinas, 79, 2007
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