vi seminário sobre pragas, doenças e plantas daninhas do ... - IAC
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Billotte N, Lagoda PJL, Risterucci AM, C. Baurens (1999) Microsatellite-enriched libraries: applied metho<strong>do</strong>logy<br />
for the development of SSR markers in tropical crops. Fruits, 54, 277–288.<br />
CIAT - Centro International de Agricultura Tropical (1990) Research constraints pro<strong>vi</strong>sionally identified by CIAT.<br />
In: Workshop on Advanced Phaseolus Bean Research network, p.30.<br />
Creste S, Tulmann-Neto A, Figueira A (2001) Detection of single sequence repeat polymorphisms in denaturing<br />
polyacrylamide sequencing gels by silver staining. Plant Molecular Biology Reporter, 19, 299-306.<br />
Kijas JMH, Fowler JCS, Garbett CA, Thomas MR (1994) Enrichment of microsatellite from the Citrus genome using<br />
biotinylated oligonucleotide sequences bound to strepta<strong>vi</strong>din-coated magnetic particles. BioTechniques, 16, 656-660.<br />
Lynch M, Walsh JB (1998) Genetics and Analysis of Quantitative Traits. Sinauer Assocs., Sunderland, MA.<br />
Silva MV da (2000) Identificação de marca<strong>do</strong>r RAPD liga<strong>do</strong> ao alelo co-7 de resistência <strong>do</strong> feijão ao agente<br />
causal da antracnose. Lavras: UFLA, 41p.<br />
Table 1. Characteristics of 20 Common bean microsatellite loci obtained from variety “<strong>IAC</strong>-UNA”, tested on 14<br />
accessions, comprising Mesoamerican and Andean gene pools; Na = number of alleles; PIC = polymorphism<br />
information content<br />
Locus<br />
Repeat motif<br />
N a<br />
Size of alelle (bp)<br />
PIC<br />
FJ 01<br />
(AC)7<br />
02<br />
270 (265-270)<br />
0.51<br />
FJ 02<br />
(AC)10 (AT)4<br />
02<br />
251 (258-260)<br />
0.52<br />
FJ 03<br />
(GT)8<br />
01<br />
178 (177)<br />
0<br />
FJ 04<br />
(AG)8<br />
01<br />
216 (218)<br />
0<br />
FJ 05<br />
(CT)2 (CA)6<br />
02<br />
212 (220-222)<br />
0.48<br />
FJ 06<br />
(GT)7<br />
0<br />
152 (*na)<br />
0<br />
FJ 07<br />
(CA)8<br />
02<br />
290 (283-297)<br />
0.52<br />
FJ 08<br />
(CA)6 (TA)3<br />
01<br />
195 (208)<br />
0<br />
FJ 09<br />
(TG)6<br />
01<br />
155 (163)<br />
0<br />
FJ 10<br />
(TG)2 (CG) (TG)4<br />
02<br />
209 (207-209)<br />
0.14<br />
FJ 11<br />
(CA)4 (C) (CA)<br />
02<br />
297 (308-310)<br />
0.25<br />
FJ 12<br />
(CA)7<br />
02<br />
256 (255-258)<br />
0.25<br />
FJ 13<br />
(AC)9<br />
02<br />
266 (267-269)<br />
0.48<br />
FJ 14<br />
(CT)8<br />
03<br />
148 (145-155)<br />
0.65<br />
FJ 15<br />
(ATGAG)4 (GT)3 … (CA)4 (CT)2<br />
02<br />
191 (200-205)<br />
0.14<br />
FJ 16<br />
(TA)6 (TG)7<br />
02<br />
284 (280-300)<br />
0.20<br />
FJ 17<br />
(TG)8 (TA)3<br />
02<br />
222 (220-230)<br />
0.48<br />
FJ 18<br />
(TG)5<br />
02<br />
224 (224-230)<br />
0.48<br />
FJ 19<br />
(CA)7<br />
02<br />
205 (210-222)<br />
0.51<br />
FJ 20<br />
(AG)3 AA (AG)3<br />
02<br />
251 (250-263)<br />
0.51<br />
Documentos, <strong>IAC</strong>, Campinas, 79, 2007<br />
160
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