vi seminário sobre pragas, doenças e plantas daninhas do ... - IAC
vi seminário sobre pragas, doenças e plantas daninhas do ... - IAC
vi seminário sobre pragas, doenças e plantas daninhas do ... - IAC
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CHARACTERIZATION OF TWENTY DINUCLEOTIDE<br />
MICROSATELLITE LOCI FOR COMMON BEAN<br />
(Phaseolus vulgaris L.)<br />
Tatiana de Campos ( 1,2 ), Luciana Lasry Benchimol ( 3 ), Sérgio Augusto Moraes Carbonell ( 3 ),<br />
Alisson Fernan<strong>do</strong> Chioratto ( 3 ), Eduar<strong>do</strong> Fernandes Formighieri ( 4 ), Anete Pereira de Souza ( 1,2 ).<br />
ABSTRACT<br />
Common bean, (Phaseolus vulgaris L.), is an importante legume food in the world. We identified and<br />
characterized twenty microssatelitte loci in common bean. An enriched library was constructed and screened<br />
for two microsatellite repeat sequences (CT and GT). Microsatellite PCR-amplification was tested for 14<br />
accesions of the <strong>IAC</strong> Germplasm Bank, including Andean and Mesoamerican gene pools. The allele<br />
number ranged from one to three, and the polimorphism information content (PIC) between 0.14 and 0.65.<br />
These polymorphic loci can be used for genetic and QTL mapping, marker-assisted selection and germplasm<br />
characterization in common bean.<br />
Keywords: Common Bean, Microsatellites, SSR-enriched libraries, molecular markers.<br />
1. INTRODUCTION<br />
Molecular markers have been used in many crop species. Common bean (Phaseolus vulgaris<br />
L.) is the principal leguminose to human consume, mainly in countries of South America and Africa<br />
(CIAT 1990). Brazil is the most important productor and consumer of bean in the world, but the crop<br />
<strong>do</strong>esn’t have the maximum producti<strong>vi</strong>ty (Silva 2000). The increase of the genetic improvement depends<br />
on the development of new molecular markers, like microsatellites.<br />
(¹) Centro de Biologia Molecular e Engenharia genética (CBMEG), UNICAMP, Campinas, SP, Brazil. E-mail: tatyuni@unicamp.br;<br />
(²) Departamento de Genética e Evolução - Instituto de Biologia (IB), UNICAMP, 13083-970, Campinas,SP, Brazil;<br />
(³) Centro de Pesquisa e Desenvol<strong>vi</strong>mento de Recursos Genéticos Vegetais, Fazenda Santa Elisa, <strong>IAC</strong>, Campinas, SP, Caixa<br />
Postal 28, 13001-970, Brazil;<br />
( 4 ) Laboratório de Genômica e Expressão, Departamento de Genética e Evolução, IB/UNICAMP, Caixa Postal 6109, 13083-970,<br />
Campinas, SP.<br />
Documentos, <strong>IAC</strong>, Campinas, 79, 2007<br />
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