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CHARACTERIZATION OF TWENTY DINUCLEOTIDE
MICROSATELLITE LOCI FOR COMMON BEAN
(Phaseolus vulgaris L.)
Tatiana de Campos ( 1,2 ), Luciana Lasry Benchimol ( 3 ), Sérgio Augusto Moraes Carbonell ( 3 ),
Alisson Fernando Chioratto ( 3 ), Eduardo Fernandes Formighieri ( 4 ), Anete Pereira de Souza ( 1,2 ).
ABSTRACT
Common bean, (Phaseolus vulgaris L.), is an importante legume food in the world. We identified and
characterized twenty microssatelitte loci in common bean. An enriched library was constructed and screened
for two microsatellite repeat sequences (CT and GT). Microsatellite PCR-amplification was tested for 14
accesions of the IAC Germplasm Bank, including Andean and Mesoamerican gene pools. The allele
number ranged from one to three, and the polimorphism information content (PIC) between 0.14 and 0.65.
These polymorphic loci can be used for genetic and QTL mapping, marker-assisted selection and germplasm
characterization in common bean.
Keywords: Common Bean, Microsatellites, SSR-enriched libraries, molecular markers.
1. INTRODUCTION
Molecular markers have been used in many crop species. Common bean (Phaseolus vulgaris
L.) is the principal leguminose to human consume, mainly in countries of South America and Africa
(CIAT 1990). Brazil is the most important productor and consumer of bean in the world, but the crop
doesn’t have the maximum productivity (Silva 2000). The increase of the genetic improvement depends
on the development of new molecular markers, like microsatellites.
(¹) Centro de Biologia Molecular e Engenharia genética (CBMEG), UNICAMP, Campinas, SP, Brazil. E-mail: tatyuni@unicamp.br;
(²) Departamento de Genética e Evolução - Instituto de Biologia (IB), UNICAMP, 13083-970, Campinas,SP, Brazil;
(³) Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Fazenda Santa Elisa, IAC, Campinas, SP, Caixa
Postal 28, 13001-970, Brazil;
( 4 ) Laboratório de Genômica e Expressão, Departamento de Genética e Evolução, IB/UNICAMP, Caixa Postal 6109, 13083-970,
Campinas, SP.
Documentos, IAC, Campinas, 79, 2007
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