visualizar - Unesp

EFFECTS OF BANDVET ON CORNEAL REPAIR OF DOGS. A
CLINICAL, MORPHOMETRICAL, HISTOCHEMISTRY
IMMUNEHISTOCHEMISTRY STUDY.
ABSTRACT - The effects of Bandvet ® on corneal repair was studied after superficial
keratectomy. Sixteen adult cross-breed dogs were used, being eight treated with
Bandvet ® (BV) and eight with saline 0.9% (CO). Daily clinical evaluations were
accomplished 3, 7, 14 and 28 days in the post-operative period. Blepharospasm,
conjuntival congestion, ocular discharge, photofobia, vascularization, pigmentation, and
corneal edema were assessed by means of clinical scores. Blepharospasm and
photofobia were noted at early stages of the post-operative period. Conjunctival
congestion and ocular discharge changed significantly at day 7 of the post-operative
period. Vascularization advanced axially in a constant manner in the early post-operative
periods, disappearing in the late stages, only in the treated group. Corneal edema was
observed in all time points in both of groups, changing significantly at day 14 of the postoperative
period. At the completion of the study, keratectomized eyes were enucleated
and corneas submitted to morphometrical evaluation (inflammatory cell count, collagen
patches count, fibroblast count, and corneal epithelium and stromal thickness
measurement) histochemistry (Alcian Blue pH 1.0 and Alcian Blue pH 2.5), digital
analysis (tridimensional chromatic evaluation of the surface), and immunohistochemistry
(fibronectin and tenascin labeling). During the 4 time points evaluated, inflammatory cell
count was increased in the BV group. An enhanced number of collagen patches were
observed in the corneas of the animals of the BV group, in the four time points
evaluated, being significant in comparison to values observed in the transitional areas
(P = 0.040). A higher fibroblast count was noted in the BV group at days 3, 14, 28, and
28. At day 7, however, the CO group showed higher cellularity. The cell count in the
transitional area changed significantly at the third day. The peripheral corneal thickness
was higher at days 3, 7 and 14, in subjects of the BV group. At day 28, however, corneal
xv