Resultados e Discussão

ABSTRACT
In this study, lipase from Burkholderia cepacia (BCL) was
firstly immobilized in starch films of taro (BCL/taro) and/or yam
(BCL/yam) and these systems were used as catalysts in the synthesis
of aroma esters derived from geraniol (22) with vinyl acetate
(4). The influence of immobilized BCL mass, reuse of the
systems and the alky chain length of propionic, butyric, hexanoic,
caprylic, capric, lauric, palmitic and stearic acids was also evaluated
in the formation of geranoil alkanoates.
The degrees of conversion into geranoil acetate, including
the reuse, were 10-87% and 17-85% using BCL/taro and
BCL/yam systems, respectively. The degrees of conversion into
geranoil alkanoates were dependent on the reaction time and
alky chain length. For instance, the values for geranoil stearate
and proprionate were 92.6% (48h) and 14.5% (24h), respectively,
using the BCL /taro system.
In another study, lipases from Candida antarctica (CALB),
B. cepacia (BCL), Aspergillus niger (ANL), Rhizopus oryzae
(ROL), Chromobacterium viscosum (VCL) and the mycelia
UEA_53 and UEA_115 in the free form or immobilized in starch
films of taro, ginger or in ginger:PEO blends were used as catalysts
in the resolution of (R,S)-1-phenylethanol (11) and some
derivatives, with vinyl or iso-propenyl acetate. Various organic
solvents (n-heptane, n-hexane, CH2Cl2, ethyl ether, acetonitrile
and 1,4-dioxane) and mixtures of n-hexane/glycerol 9:1 (v/v) or nhexane/[BMIm][PF6]
9:1 (v/v), were screened as reaction media.
The conversion into (R)-(+)-1-phenylethyl acetate (R)-(+)-
12 was dependent on the molar ratio of vinyl or iso-propenyl acetate.
In the reaction with vinyl acetate the conversion degrees
were 8.7-22.5% using the BCL/taro system and 7.7-24.1% using
the BCL/ginger system. Using iso-propenyl acetate, the conversion
degrees were 4.1 (24 h) and 12.6% (96 h) with the
BCL/ginger system. Using molar ratios of (R,S)-11:4 of 1:1, 1:2
and 1:5, the values of eep and E were > 99% and > 200, respectively.
Using the lipases ROL, ANL, VCL, BCL, CALB and the
mycelia UEA_53 and UEA_115 in the free form, the conversion
degrees to the (R)-(+)-12 ester were 0.2 - 56.0%, eep was 85-
99% and E was 39-200. Using the immobilized lipases ROL,
ANL, VCL and BCL the highest conversion degree was 13% (r.t)
xix