Tese Michele Fagundes.pdf - Caunesp

Abstract
With the growth of aquaculture, a gretaer number of fish is handled in the
stations, but the farming practice can be traumatic to animals IF it were not done with
the appropriate handling. One of the techniques utilized to minimize the fish stress is the
use of anesthetics. The aim of this work was to test the efficience of three anesthetics
(benzocaine, phenoxyethanol and cloeve oil) in pintado (Pseudoplatystoma corruscans).
Fish were exposed to three anesthetic (25, 50 and 75 mg/L benzocaine; 0.2, 0.4 and 0.8
mL/L of phenoxyethanol and clove oil). Blood cortisol levels reduced as the eugenol
concentration increased, and the same profile was observed with phenoyethanol. No
difference was observed regarding benzocaine. Similarly, glucose reduced with the
anesthetics increased, being the lower levels found in the lower concentrations of both
anesthetics. Similar profile of reduction was seen regarding to benzocaine and eugenol
in hematocrit, number of red cells and hemoglobin. In all three anesthetics, the lowest
concentrations promoted the higher time of anesthetic induction. The recovery time did
not differ between benzocaine and phenoethanol, but colve oil showed the highest times
at the highest concentrations. All anesthetics tested induced surgical state of anesthesia
and concentrations of 75mg/L benzocaine and 0.8 mL/L 2-phenoxyethanol and clove oil
presented appropriate times of anesthetic induction and recovery at the same time of the
lowest blood levels of cortisol and glucose.
Key words: Pintado (Pseudoplatystoma corruscans), benzocaine, 2-phenoxyethanol,
clove oil, cortisol, anesthesia, fish
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