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entendimento dos mecanismos envolvidos na instalação e na manutenção da dor neuropática.
ABSTRACT Brachial plexus avulsion (BPA) in mice (Swiss, C57/BL6 and J129) produced long- lasting mechanical and thermal sensitization, which were evaluated in the right hindpaw and in the tail. These altered sensations were not observed in the operated paw. The animals also did not show a sickness behaviour characterized by weight loss and decrease of locomotor activity. Data obtained with knockout TNFα p55 -/- mice, antibody anti-TNFα or thalidomide demonstrated that TNFα has a fundamental role in the onset of mechanical and thermal hypernociception. Furthermore, it was verified, by using antibodies against neurotrophic factors, that NGF, GDNF, BDNF and NT-3 have a role earlier in the process of mechanical hypernociception induced by BPA, probably in the sensitization of spinal sensory neurones. On the other hand, only BDNF seemed to have importance for the maintenance of hypernociceptive responses in this model. B1 kinin receptor, but not B2 kinin receptor, appeared to play a significant role in the genesis of hypernociceptive responses, confirmed by the use of knockout mice for each receptor (B1 or B2). Moreover, B1 receptor has an important participation on the maintenance of these sensory alterations using selective antagonists. We suggested here that the mediators cited above, and the B1 receptor seem to initiate a cascade of events responsible for the hypernociceptive state in this neuropathic pain model. On the other hand, the neurotrophic factor BDNF and B1 kinin receptor seem to be fundamentally related to the maintenance of mechanical hypernociception induced by BPA in mice. These results together, collaborate for better understand the mechanisms involved on the onset and on the maintenance of neuropathic pain.
Page 1 and 2: NARA LINS MEIRA QUINTÃO ANÁLISE D
Page 3 and 4: Depois de algum tempo você.... ...
Page 5 and 6: Às minhas amigas Ali, Beth, Gica,
Page 7 and 8: hipernociceptivas induzidas pela AP
Page 9 and 10: LISTA DE ABREVIAÇÕES aa Aminoáci
Page 11 and 12: TBE Tris/borato/EDTA TNFα Fator de
Page 13 and 14: Figura 18 - Efeito dos anticorpos c
Page 15: RESUMO A avulsão do plexo braquial
Page 19 and 20: através das vias sensoriais e, a n
Page 21 and 22: podendo persistir até mesmo após
Page 23 and 24: de estímulo e, após sensibilizaç
Page 25 and 26: Figura 1. Vias envolvidas na dor ne
Page 27 and 28: dos efeitos antidepressivos, pois e
Page 29 and 30: técnicas modernas de farmacologia,
Page 31 and 32: associada à dor neuropática, pouc
Page 33 and 34: 1997). Segundo Wynn Parry (1980), a
Page 35 and 36: 2003). Por outro lado, os fatores n
Page 37 and 38: “neurotrófico” é aplicada ao
Page 39 and 40: A neurotrofina NT-3 exerce um papel
Page 41 and 42: As cininas desempenham seus efeitos
Page 43 and 44: 2. OBJETIVOS 2.1. Objetivo geral Ad
Page 45 and 46: 3. MATERIAL E MÉTODOS 3.1. Animais
Page 47 and 48: 3.3. Análise do limiar mecânico a
Page 49 and 50: 1957). O aparelho de rota-rod (Ugo
Page 51 and 52: (0,5 µg/sítio) ou i.c.v. (0,5 µg
Page 53 and 54: anteriormente em diferentes interva
Page 55 and 56: utilizando os seguintes primers: se
Page 57 and 58: PBS (todos da Sigma Chemical Compan
Page 59 and 60: Figura 5 - Hipernocicepção mecân
Page 61 and 62: 4.3. Peso corporal, atividade locom
Page 63 and 64: 4.4. Análise dos diferentes mecani
Page 65 and 66: 4.4.1.2. Efeito do anticorpo anti-T
Page 67 and 68:
4.4.1.3. Efeito do anticorpo anti-T
Page 69 and 70:
Figura 12 - Efeito dos anticorpos c
Page 71 and 72:
Figura 13 - Efeito dos anticorpos c
Page 73 and 74:
Quando os camundongos receberam uma
Page 75 and 76:
Do mesmo modo, a administração po
Page 77 and 78:
É válido ressaltar que o tratamen
Page 79 and 80:
4.4.3.1. Efeito do antagonista sele
Page 81 and 82:
Figura 22 - Hipernocicepção mecâ
Page 83 and 84:
Figura 23 - Efeito de antagonistas
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
A B Figura 26 - Quantificação do
Page 91 and 92:
5. DISCUSSÃO Por séculos, médico
Page 93 and 94:
envolvidas nestes dois tipos de hip
Page 95 and 96:
epresentar a solução para a compr
Page 97 and 98:
Como se pode observar no presente e
Page 99 and 100:
Recentemente, estas proteínas têm
Page 101 and 102:
mediadores (tais como BK e SP), é
Page 103 and 104:
induzidas pela APB, embora seu envo
Page 105 and 106:
Após axotomia, os neurônios corti
Page 107 and 108:
densidade moderada de sítios de li
Page 109 and 110:
Pesquero e colaboradores (2000) des
Page 111 and 112:
lot, confirmam a hipótese do envol
Page 113 and 114:
eduzindo o limiar do reflexo nocice
Page 115 and 116:
Figura 28. Proposta para os possív
Page 117 and 118:
APFEL, S.C.; WRIGHT, D.E.; WIIDEMAN
Page 119 and 120:
BOUCHER, T.J.; OKUSE, K.; BENNETT,
Page 121 and 122:
the brain is increased during persi
Page 123 and 124:
FERREIRA, J.; BEIRITH, A.; MORI, M.
Page 125 and 126:
GUIMARÃES, J.A.; BORGES, B.R.; PRA
Page 127 and 128:
JOHNSON, D.; LANAHAN, A.; BUCK, C.R
Page 129 and 130:
LOESER, J.D.. Pain and suffering. C
Page 131 and 132:
their central terminations within t
Page 133 and 134:
POO, M.. Neurotrophins as synaptic
Page 135 and 136:
SANG, C.N.. NMDA-receptor antagonis
Page 137 and 138:
SOMMER, C.; PETRAUSCH, S.; LINDENLA
Page 139 and 140:
WALL, P.D.; SCADDING, J.W.; TOMKIEW
Page 141 and 142:
7. ANEXOS
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