SodininkyStĖ ir darŽininkyStĖ 27(3)

SODININKYSTĖ IR DARŽININKYSTĖ. SCIENTIFIC ARTICLES. 2008. 27(3).Investigation of cold hardiness of agriculture plants in vitroR. Rugienius, V. Lukoševičiūtė, G. Stanienė, V. StanysSummaryResults of strawberry, rape, cydonia, miscanthus and other important crops cold hardinessinvestigations are reviewed in this article. The main task of these investigations was toestablish the optimal conditions for plant genotype differentiation of these species by coldhardiness, evaluate factors affecting cold acclimation and its persistence. According the dataof most investigations, for genotype cold hardiness differentiation cold acclimation is essential.Freezing in temperatures -2–25 °C was applied in the studies. It was shown that optimalfreezing temperature for differentiation of most genotypes by cold hardiness in vitro is inrange between -6 and -12 °C. It was revealed that plants of various genotypes react to freezingin vitro very differently. Great impact on plant cold hardiness causes various additions ofgrowing media – sucrose, vitamins, phytohormons and ectr. It was shown that cold acclimationpersistence during “thaws” depends on temperature, phytohormon content in the plant ormedium and genetic background of the cultivar. Freezing in vitro could be applied not only forplant genotype differentiation, but in the investigations of cold hardiness mechanisms as well.Key words: strawberry, miscanthus, Cydonia, freezing in vitro, cultivars, rape.59