SodininkyStĖ ir darŽininkyStĖ 28(2)

12. Tang H., Ren Z., Reustle G., Krczal G. 2002. Plant regeneration from leaves ofsweet and sour cherry cultivars. Scientia Horticulturae, 93(3–4): 235–244.13. Vardja R., Vardja T. 2004. Agrobacterium rhizogenes-mediated transformationand regeneration of bisecsual Actinidia kolomikta. Acta universitatis Latviensis,Biology, 676: 247–250.14. Zhu L. H., Holefors A., Ahlman A., Xue Z.T., Welander M. 2001. Transformationof the rootstock M.9/29 with the rolB gene and its influence on rooting andgrowth. Plant Sci. 160: 433–443.15. Полевой В. В. 1989. Физиология растений. Высшая школа, Москва.SODININKYSTĖ IR DARŽININKYSTĖ. SCIENTIFIC ARTICLES. 2009. 28(2)Regeneration of different aronia explants in vitro depending oncytokinin and antibioticsD. Gelvonauskienė, J. Vinskienė, T. Šikšnianas, V. Bendokas,I. Stepulaitienė, G. Stanienė, V. StanysSummarySystem for regeneration of aronia (Aronia melanocarpa) in vitro after transformation usingbiobalistic and Agrobacterium tumefaciens methods was prepared in the Plant BiotechnologyLaboratory of the Lithuanian Institute of Horticulture. It was estimated, that the most suitableexplants for regeneration of aronia in vitro are microshoots and hypocotyls, because theyhave large morphogenesis in vitro potential. Optimal conditions for regeneration in vitro areas follows – MS nutritional medium with addition of 0.3 µM NAR and 10 µM TDZ (pH 5.8),induction of regeneration for 6 weeks in the dark, after the explants are grown in 16 hourphotoperiod for 6 weeks. The lowest effective concentration of kanamycin for identificationof regenerated transformants of aronia was 15 mg l -1 , and 250 or 500 mg l -1 concentrationof cefotaxim in nutritional medium was the most effective for elimination of agrobacteria.Key words: aronia, cefotaxim, explant, in vitro, kanamycin, regeneration, tidiazuron.54