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SODININKYSTĖ IR DARŽININKYSTĖ. SCIENTIFIC ARTICLES. 2009. <strong>28</strong>(2).Effect of co-cultivation with Agrobacterium tumefaciens on in vitroregeneration of winter rapeseedN. Burbulis, R. Kuprienė, A. Blinstrubienė, V. JonytienėSummaryThe investigations were carried out in the Agrobiotechnology Laboratory of LithuanianUniversity of Agriculture. Aim of the research – to evaluate effect of co-cultivation of theexplant with agrobacterium, containing the different plasmids, on regeneration responseof winter rapeseed. Stem segments of Brassica napus co-cultivated with Agrobacteriumtumifaciens GV3101 strain containing the plasmids pART-BnMYBsn-MYBanti, pHellsgate-BnCBF and pHellsgate-BnICE. The results of the experiments indicated a difference amongthe tested cultivars regarding the<strong>ir</strong> organogenic response depended on genotype and vectorconstruction. Explants inoculation with agrobacterium containing different plasmids didn’thave any significant effect on adventitious shoots formation in cultivars ‘Libea’, ‘Liprima’(pART-BnMYBsn-MYBanti and pHellsgate-BnICE) and cultivar ‘Siska’ (pHellsgate-BnICE).The co-cultivation of explants with A. tumefaciens had a significant negative influence onshoot formation frequency of other tested cultivars. Kanamycin-resistant plant-regenerantsformation frequency strongly depended on genotype-plasmid interaction. Highest amountof resistant regenerants have been obtained after inoculation with A. tumefaciens containingpHellsgate-BnICE (cv. ‘Banjo H’); pHellsgate-BnCBF (cvs. ‘Elvis’, ‘Valesca’, ‘Libea’,‘Liprima’ and ‘Insider’); pART-BnMYBsn-MYBanti (cvs. ‘Silvia’ and ‘Siska’).Key words: Agrobacterium tumifaciens, Brassica napus, co-cultivation, kanamycinresistance,organogenesis.171
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