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ref. 6562866 pathogenic system double - Liofilchem

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*PATHOGENIC SYSTEM DoubleRESULTSTEST PROCEDUREIdentificationAB1PYR2SGA*1PYR2SGAStreptococcus pyogenes (Group A)* PYR test agglutination test<strong>Liofilchem</strong> ® and the <strong>Liofilchem</strong> company logo are registred trademarks of LIOFILCHEM s.r.l.5ESC8PSE3STA4HAE6IND7PRO11STR3STA* **9OX10KES12CAN5ESC8PSE4HAE6IND7PRO*9OX10KES11STR12CANStaphylococcus aureus agglutination test* coagulase testHaemophylus spp.- confirm by culture mediumEcherichia coli* Indole testProteus/Providencia spp.Pseudomonas spp.* Oxidase testKlebsiella/Enterobacter/Serratia spp.- confirm by culture mediumEnterococcus spp. (Group D)Candida spp.* confirm at the microscope1 2Dip the swab of the specimeninto a tube of nutrient broth andincubate at 36±1°Cfor 2-4-6 hours.Transfer 0.5 mL of culture brothinto the vial of PhysiologicalSolution (Specimen Suspension).3 4Add two drops of PYR Reagentinto the well 1-PYR. The pink-redcolouring of the disc within 5minutes identifies Streptococcuspyogenes (Group A).Confirm the presence ofS. pyogenes (Group A) by Strepto Alatex kit.Add a Pyr Disc into the well1-PYR.Add 0.2 mL of the SpecimenSuspension into each well of the<strong>system</strong>, add one drop of vaselineoil into the well 10-UR.ncubate at 36±1°C for 18-24 h.Confirm the presence ofStaphylococcus aureus by Staphlatex kit or coagulation test.Confirm the presence ofHaemophylus spp. byinoculating a selectiveculture medium.DESCRIPTION PACKAGING REF.PATHOGENIC SYSTEMKOVAC’S - REAGENTVASELINE OILOXIDASE TEST DISCS40 tests4X25 mL4X50 mL30 discs71678802718027988004STREPTO A LATEX KITSTAPH LATEX KIT50 tests50 tests96154960175 6Confirm the presence ofEscherichia coli adding two dropsof Kovac’s Reagent into the well6-IND (Indole Test).Confirm the presence ofPseudomonas spp. adding a disc ofOxidase test into the well 9-OX.Confirm Candida spp. by takinga drop from the positive well12-CAN and watch for spores atthe microscope (400x).Note down the test results onthe form provided in the kit, orread and interpret by automaticreaders.64026 Roseto degli Abruzzi, ItalyTel. : +39 0858930745Fax : +39 0858930330www.liofilchem.net<strong>ref</strong>. <strong>6562866</strong> - Rev. 2/ 22-10-2012© <strong>Liofilchem</strong> 2012 Printed in Italy

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