scienze della vita roma, 22-23 ottobre 2012 - SIF

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VALIDATION OF THE MBS METHOD 1 Giorgia Bottini, 1 Francesca Losito, 1 Alyexandra Arienzo, 2 Francesca Romana Priolisi, 2 Alberto Mari and 1 Giovanni Antonini 1 Department of Biology, University Roma Tre, 00146 Rome, Italy 2 MBS srl, 00131 Rome, Italy Corresponding Author: Prof. Giovanni Antonini, Department of Biology, University Roma Tre, 00146 Rome, Italy, Tel: +39-3290570913; E-mail: giovanni.antonini@uni<strong>roma</strong>3.it Food borne pathogens are a growing concern for human safety. In this context, MBS srl (a spin-off of Roma Tre University, Rome, Italy) has developed an alternative method, called Micro Biological Survey (MBS) method. The MBS method is a rapid alternative method for the detection and selective counting of bacteria in agro-food, in water and in environmental samples. It is based on colorimetric survey performed in mono-use disposable reaction vials in which samples can be inoculated without any preliminary treatment (eg. homogenization, dilution, etc.); the greater the number of bacteria in the sample, the faster the color of the reaction vial changes. The objective of this study was the primary validation, in accord with ISO 16140 (2003), of the qualitative Micro Biological Survey (MBS) method for Salmonella spp. and Listeria spp. and the quantitative Biological Survey (MBS) method for Total Viable Count (TVC), Escherichia coli (E. coli), Enterobacteriaceae and Staphylococcus aureus. Validation aims to compare the results obtained with an alternative method, in this case the MBS method, with the results obtained with the reference method. To verify the equivalence between the two methods different parameters were analyzed: selectivity, relative accuracy, relative specificity and relative sensitive for the qualitative validation of the MBS method for Salmonella spp. and Listeria spp.; selectivity, linearity and accuracy for the quantitative validation of the MBS method for Enterobacteriaceae and Staphylococcus aureus. The validation has shown that the MBS method gives similar results and is in agreement with the reference methods. The MBS method can therefore represent a worthy aid in food screening without replacing the analysis carried out with traditional methods which are very precise though often long and complex. 68
UNITÀ DI RICERCA DEL CONSORZIO INTERUNIVERSITARIO “ISTITUTO NAZIONALE BIOSTRUTTURE E BIOSISTEMI” (in ordine alfabetico del nome del Responsabile Scientifico) (PO Professore Ordinario, PA Professore Associato, RU Ricercatore Universitario, DR Dottorando di Ricerca;BC Borsista, Contrattista; PT Personale Tecnico; A Altro) 69
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CONSORZIO INTERUNIVERSITARIO X CONV
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INDICE Programma X Convegno Naziona
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PROF.SSA ADRIANA MAGGI - Univ. MI
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RELAZIONE INTRODUTTIVA E’ la prim
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quinto e sesto posto ed hanno un nu
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ABSTRACT DELLE COMUNICAZIONI SCIENT
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REDOX REGULATION OF CELLULAR STRESS
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Page 21 and 22: Sessione “Nanobiotecnologie” 21
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Page 41 and 42: Marco Biglietto AB SCIEX Italy PUSH
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Page 45 and 46: UVI5008: UN TRIPLO INIBITORE EPIGEN
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� Della Torre S, Rando G, Meda C,
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RESPONSABILE SCIENTIFICO MAGNAGHI V
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RESPONSABILE SCIENTIFICO: MARINO MA
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RESPONSABILE SCIENTIFICO MAIOLI MAR
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UNITA’ DI RICERCA INBB Genova RES
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RESPONSABILE SCIENTIFICO MIGHELI QU
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RESPONSABILE SCIENTIFICO OLMO ETTOR
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RESPONSABILE SCIENTIFICO: RIGO ADEL
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RESPONSABILE SCIENTIFICO RODA ALDO
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PRINCIPALI ATTREZZATURE DI CUI DISP
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- Speciale A, Chirafisi J, Saija A,
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3) A.L. Oliveira, M. Gallo, L. Pazz
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9. Cataldi M, Panuccio G, Cavaccini
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L. Vergani, C. Lanza, P. Rivaro, M.
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scienze della vita roma, 22-23 ottobre 2012 - SIF

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