Recovery Studies for QuEChERS

Recovery Studies using the QuEChERS Method*M. AnastassiadesFortification level was 0.1 mg/kg in all cases. Calibrations were performedusing matrix-matched standards. RSDs (in %) ranged between 1-15; usuallybelow 5 (n=5).In the following table includes pesticides for which recoveries were between70 and 110 %. In most cases, the recoveries ranged between 90 and 100 %.*publication in preparationPesticide Commodity Determination Cleanupwith PSANocleanupRemarks2,3,6-Trichlorobenzoic acid Apple LC-MS/MS x pH up to 6avoid PSA2,4,5-T Apple LC-MS/MS x pH up to 6avoid PSA2,4,5-TP Apple LC-MS/MS x pH up to 6avoid PSA2,4-D Apple LC-MS/MS x pH up to 6avoid PSA2,4-DB Apple LC-MS/MS x pH up to 6avoid PSA2,4-Dichlorobenzoic acid Apple LC-MS/MS x pH up to 6avoid PSA2,4-DP Apple LC-MS/MS x pH up to 6avoid PSA2,6-Dichlorobenzamide Apple LC-MS/MS x xCucumber LC-MS/MS x2-Naphthyloxyacetic acid Apple LC-MS/MS x pH up to 6avoid PSA2-PhenylphenolApple GC-MSD x xCucumber GC-MSD x x4-CPA Apple LC-MS/MS x pH up to 6avoid PSAAcephateApple LC-MS/MS x xCucumber LC-MS/MS x xAcetamipridApple LC-MS x xCucumber LC-MS x xAcrinathrinApple GC-MSD x xCucumber GC-MSD x xAldicarbApple LC-MS x xCucumber LC-MS x xAminocarbApple LC-MS x xCucumber LC-MS x xAmitrazApple LC-MS/MS xCucumber LC-MS/MS x xPage 1 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksAtrazineAtrazine-desethylAtrazine-desisopropylApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xAzinphos-methyl Cucumber GC-MSD x x Sensitive towardsdecompositionin the injectorAzoxystrobinApple GC-MSD x xCucumber GC-MSD x xBeflubutamidApple GC-MSD x xCucumber GC-MSD x xBenalaxylApple GC-MSD x xCucumber GC-MSD x xBenazolin Apple LC-MS/MS x pH up to 6avoid PSABendiocarbApple LC-MS x xCucumber LC-MS x xBentazone Apple LC-MS/MS xBifenazateApple GC-MSD x xCucumber GC-MSD x xBifenthrinApple GC-MSD x xCucumber GC-MSD x xBiphenylApple GC-MSD x xCucumber GC-MSD x xBitertanolApple GC-MSD x xCucumber GC-MSD x xBoscalid (Nicobifen) Apple LC-MS/MS x xCucumber LC-MS/MS x xBromacil Apple LC-MS/MS xBromoxynil Apple LC-MS/MS xBrompropylateApple GC-MSD x xCucumber GC-MSD x xBromuconazoleApple GC-MSD x xCucumber GC-MSD x xBupirimateApple GC-MSD x xCucumber GC-MSD x xBuprofezinApple GC-MSD x xCucumber GC-MSD x xCaptan Apple GC-MSD x x Sensitive towardsdecomposition **Page 2 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksCarbarylCarbendazimCarbetamideCarbofuranCadusafosChlorfenvinphosChlorfluazuronChloridazonChlorpyrifosChlorpyrifos-methylChlorothalonilChlorotoluronChlozolinateChromafenozideClomazoneApple LC-MS x xCucumber LC-MS x xApple LC-MS x xCucumber LC-MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS x xCucumber LC-MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD xCucumber GC-MSD x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xSensitive towardsdecomposition **Clopyralid Apple LC-MS/MS x pH up to 5avoid PSACyfluthrinApple GC-MSD x xCucumber GC-MSD x xCyhexatinApple LC-MS xCucumber LC-MS xCypermethrinApple GC-MSD x xCucumber GC-MSD x xCyproconazolApple GC-MSD x xCucumber GC-MSD x xPage 3 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksCyprodinilApple GC-MSD x XCucumber GC-MSD x xCyromazine Apple LC-MS x xDDD opDDD ppDDE opDDE ppDDT opDDT op + DDD ppDDT ppDeltamethrinDemeton-S-methylDemeton-S-methylsulfonDiazinonApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xSame retention timeand target massesDicamba Apple LC-MS/MS 97,1 pH up to 5avoid PSADichlofluanidApple GC-MSD x x Sensitive towardsCucumber GC-MSD x xdecomposition **DichlorobenzophenoneApple GC-MSD x xpp'Cucumber GC-MSD x xDichlorvosApple GC-MSD x xCucumber GC-MSD x xDiclobutrazolApple GC-MSD x xCucumber GC-MSD x xDicloranApple GC-MSD x xCucumber GC-MSD x xDicofol Apple GC-MSD x Sensitive towardsdecomposition **Page 4 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksDicrotophosDieldrinDiethofencarbDifenconazoleDiflubenzuronDiflufenicanDimefuronDimethachlorDimethenamidDimethoateDimethomorphDiniconazoleDinocapDioxacarbDiphenylamineDisulfotonDiuronEndosulfan alphaEndosulfan betaEndosulfan sulfateApple LC-MS/MS x xCucumber LC-MS/MS x XApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD xCucumber GC-MSD x xApple LC-MS xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xProblems withdecomposition at low pHPage 5 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksEndrinEpoxiconazoleEsfenvalerateEthiofencarbEthionEthirimolEthoprophosEtofenproxEtridiazoleEtrimfosFamoxadoneFenbutatin oxideFenamiphosFenarimolFenbuconazoleFenhexamidFenitrothionFenobucarbFenoxycarbFenpropathrinApple GC-MSD x xCucumber GC-MSD x XApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xPage 6 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksFenpropidinFenpropimorphFensonFenthionFenthion-sulfoxideFentinFenvalerateApple GC-MSD xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xFluazifop Apple LC-MS/MS x pH up to 6avoid PSAFluazinamApple GC-MSD x xCucumber GC-MSD x xFluazuronApple LC-MS x xCucumber LC-MS x xFlucythrinateApple GC-MSD x xCucumber GC-MSD x xFludioxonilApple GC-MSD x xCucumber GC-MSD x xFlufenacetApple LC-MS/MS x xCucumber LC-MS/MS x xFlufenoxuronApple LC-MS x xCucumber LC-MS x xFluquinconazoleApple GC-MSD x xCucumber GC-MSD x xFluroxypyr Apple LC-MS/MS x Avoid PSAFlusilazoleApple GC-MSD x xCucumber GC-MSD x xFolpetApple GC-MSD x x Sensitive towardsCucumber GC-MSD xdecomposition **FonofosApple GC-MSD x xCucumber GC-MSD x xHalofenozideApple LC-MS x xCucumber LC-MS x xHexachlorobenzene Apple GC-MSD x xCucumber GC-MSD x xPage 7 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksHCH alphaHCH betaHeptachlorHeptachlor epoxidecisHeptachlor epoxidetransHexaconazoleHexaflumuronHexazinoneHexythiazoxImazalilApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD xCucumber GC-MSD xApple LC-MS x xCucumber LC-MS x xImazapyr Apple LC-MS/MS x pH up to 5avoid PSAImazaquin Apple LC-MS/MS x pH up to 6avoid PSAImazethapyr Apple LC-MS/MS x pH up to 5avoid PSAImidaclopridApple LC-MS x xCucumber LC-MS x xIndoxacarbApple GC-MSD x xCucumber GC-MSD x xIoxynil Apple LC-MS/MS xIprodioneApple GC-MSD x xCucumber GC-MSD x xIsofenphosApple GC-MSD x xCucumber GC-MSD x xIsoprocarbApple LC-MS x xCucumber LC-MS x xIsoproturonApple LC-MS/MS x xCucumber LC-MS/MS x xKresoxim-methylApple GC-MSD x xCucumber GC-MSD x xPage 8 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksLambda-CyhalothrinLandrinLenacilLindaneLinuronLufenuronMalaoxonMalathionApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD xCucumber GC-MSD xApple GC-MSD x xCucumber GC-MSD x xMCPA Apple LC-MS/MS x pH up to 6avoid PSAMCPB Apple LC-MS/MS x Avoid PSAMecarbamApple GC-MSD x xCucumber GC-MSD x xMecoprop Apple LC-MS/MS x pH up to 6avoid PSAMepronilApple GC-MSD x xCucumber GC-MSD x xMetalaxylApple GC-MSD x xCucumber GC-MSD x xMetamitronApple LC-MS/MS x xCucumber LC-MS/MS x xMetazachlorApple GC-MSD x xCucumber GC-MSD x xMethabenzthiazuron Apple LC-MS/MS x xCucumber LC-MS/MS x xMethamidophosApple LC-MS/MS x xCucumber LC-MS/MS x xMethidathionApple GC-MSD x xCucumber GC-MSD xMethiocarbApple LC-MS x xCucumber LC-MS x xMethomylApple LC-MS x xCucumber LC-MS x xPage 9 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksMethoxychlorApple GC-MSD x Sensitive towardsCucumber GC-MSD xdecompositionin the injectorMethoxyfenozideApple LC-MS x xCucumber LC-MS x xMetobromuronApple LC-MS/MS x xCucumber LC-MS/MS x xMetolachlorApple LC-MS/MS x xCucumber LC-MS/MS x xMetolcarbApple LC-MS x xCucumber LC-MS x xMetosulamApple LC-MS/MS x xCucumber LC-MS/MS x xMetoxuronApple LC-MS/MS x xCucumber LC-MS/MS x xMetribuzinApple LC-MS/MS x xCucumber LC-MS/MS x xMevinphosApple GC-MSD x xCucumber GC-MSD x xMonocrotophosApple LC-MS-MS x xCucumber LC-MS-MS x xMyclobutanilApple GC-MSD x xCucumber GC-MSD x xNaphthylacetic acid Apple LC-MS/MS x pH up to 6avoid PSANitenpyramApple LC-MS x xCucumber LC-MS x xNuarimolApple GC-MSD x xCucumber GC-MSD x xOfuraceApple GC-MSD x xCucumber GC-MSD x xOmethoateApple LC-MS/MS x xCucumber LC-MS/MS x xOxadiazonApple GC-MSD x xCucumber GC-MSD x xOxadixylApple GC-MSD x xCucumber GC-MSD x xOxamylApple LC-MS x xCucumber LC-MS x xOxydemeton-methyl Apple LC-MS-MS x xCucumber LC-MS-MS x xPage 10 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksParaoxonParaoxon-methylParathionParathion-methylPenconazolePencycuronPermethrinPhoratePhosalonePhosmetPhosphamidonApple GC-MSD xCucumber GC-MSD xApple GC-MSD xCucumber GC-MSD xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD xCucumber GC-MSD xApple GC-MSD xCucumber GC-MSD xPicloram Apple LC-MS/MS x pH up to 5avoid PSAPiperonyl butoxide Apple GC-MSD x xCucumber GC-MSD x xPirimicarbApple GC-MSD x xCucumber GC-MSD x xPirimiphos-methyl Apple GC-MSD x xCucumber GC-MSD x xProchlorazApple LC-MS x xCucumber LC-MS x xProcymidoneApple GC-MSD x xCucumber GC-MSD x xProfenofosApple GC-MSD x xCucumber GC-MSD x xPromecarbApple LC-MS x xCucumber LC-MS x xPrometrynApple LC-MS/MS x xCucumber LC-MS/MS x XPage 11 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksPropamocarbPropargitePropazinPropiconazolePropoxurPropyzamideProthiofosPymetrozinePyraclostrobinPyrazophosPyridabenPyridaphenthionPyrifenoxPyrimethanilPyriproxyfenQuinalphosQuinoxyfenQuintozeneQuizalofop-ethylSebuthylazineApple LC-MS xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xGC-MSDApple LC-MS-MS x xCucumber LC-MS-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS/MS x xCucumber LC-MS/MS x xDegradationat low pHPage 12 / 13 2004-01-30

Pesticide Commodity Determination Cleanupwith PSANocleanupRemarksSimazineSpiroxamineSulfotepTau-FluvalinateTebuconazoleTebufenozideTebufenpyradTeflubenzuronTerbufosTerbuthylazinTerbuthylazin-desethylTerbutrynTetraconazoleTetradifonThiabendazoleThiaclopridThiometonTolclofos-methylApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple LC-MS/MS x xCucumber LC-MS/MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber LC-MS x xApple LC-MS x xCucumber LC-MS x xApple GC-MSD x xCucumber GC-MSD x xApple GC-MSD x xCucumber GC-MSD x x** Degradation at various stages of analysis including storage of extracts and GC-injection. Most vulnerableat elevated pH valuesPage 13 / 13 2004-01-30