ALLERGEN Total IgE REF - Radim S.p.A.
ALLERGEN Total IgE REF - Radim S.p.A.
ALLERGEN Total IgE REF - Radim S.p.A.
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SCHEMA DEL DOSAGGIO - ASSAY SCHEME - ASSAYSCHEMA<br />
Pozzetti<br />
Wells<br />
CAL<br />
Campioni,<br />
Samples<br />
Reag.<br />
Proben<br />
BUF│INC 150 µl 150 µl<br />
CAL 25 µl ----<br />
Campioni, Samples, Proben ---- 25 µl<br />
− Incubare / Incubate / Inkubieren: 60 min., 37°C<br />
− Aspirare e Lavare / Aspirate and Wash / Absaugen und Waschen: 3 x 350 µl.<br />
CONJ│BIOT 100 µl 100 µl<br />
− Incubare / Incubate / Inkubieren: 30 min., 37°C<br />
− Aspirare e Lavare / Aspirate and Wash / Absaugen und Waschen: 3 x 350 µl.<br />
CONJ│HRP 100 µl 100 µl<br />
− Incubare / Incubate / Inkubieren: 30 min., 37°C<br />
− Aspirare e Lavare / Aspirate and Wash / Absaugen und Waschen: 3 x 350 µl.<br />
SUBS│TMB 100 µl 100 µl<br />
− Incubare / Incubate / Inkubieren: 15 min., T.A./RT (20-25°C)<br />
STOP 100 µl 100 µl<br />
− Leggere / Read / Messen: 450-405 nm.<br />
A1002 – <strong>ALLERGEN</strong> <strong>Total</strong> <strong>IgE</strong><br />
M541 – Rev. 1 – 06/2010 – Pag. 30/32
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