CagA IgG EIA WELL REF K6HPG - Radim S.p.A.
CagA IgG EIA WELL REF K6HPG - Radim S.p.A.
CagA IgG EIA WELL REF K6HPG - Radim S.p.A.
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8. SCHEMA DEL DOSAGGIO - ASSAY SCHEME<br />
Prediluizione del campione, Sample predilution: 1/300<br />
Pozzetti<br />
Wells<br />
Bianco<br />
Blank<br />
CAL Campioni<br />
Samples<br />
(dil.)<br />
Reag.<br />
CAL ----- 100 µL -----<br />
Campioni (diluiti)<br />
Diluted Samples<br />
----- ----- 100 µL<br />
DIL 100 µL ----- -----<br />
− Incubare, Incubate: 37±2°C, 60±5 min.<br />
− Aspirare e Lavare, Aspirate and wash: 4 x 350 µL.<br />
CONJ 100 µL 100 µL 100 µL<br />
− Incubare, Incubate: 37±2°C, 30±2 min.<br />
− Aspirare e Lavare, Aspirate and wash: 4 x 350 µL.<br />
TMB 100 µL 100 µL 100 µL<br />
− Incubare, Incubate: 37±2°C, 15'<br />
STOP 100 µL 100 µL 100 µL<br />
− Leggere, Read: 450-405 nm.<br />
<strong>K6HPG</strong> –<strong>CagA</strong> <strong>IgG</strong> <strong>EIA</strong> <strong>WELL</strong><br />
M197 – Rev.9 – 06/2008 – Pag. 23/ 24
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