遺伝子発現時系列の システム論的解析 - Computational Biology ...

20013
20014
20044
CBRC
CBRC
DNA

20013
20014
20044
CBRC
CBRC
DNA

2001 2002 2003 2004 2005

1.
2001-2005

1.
http://www.genome.jp/kegg/pathway/hsa/hsa04210.html
=

1.
=
DNA
S-system
Tominaga(03), Kikuchi(03)

1.
S-system
dX i
dt
n+m
∏
= α i
j=1
X g ij
j
n+m
∏
− β i
j=1
X h ij
j
X1
g21 h21
X2
&

1.
GA)
Scale-free

1.
(Pentium III 867MHz × 64)
i α i g i1 g i2 g i3 g i4 g i5 β i h i1 h i2 h i3 h i4 h i5
1 5.0 0.0 0.0 1.0 0.0 -1.0 10.0 2.0 0.0 0.0 0.0 0.0
2 10.0 2.0 0.0 0.0 0.0 0.0 10.0 0.0 2.0 0.0 0.0 0.0
3 10.0 0.0 -1.0 0.0 0.0 0.0 10.0 0.0 -1.0 2.0 0.0 0.0
4 8.0 0.0 0.0 2.0 0.0 -1.0 10.0 0.0 0.0 0.0 2.0 0.0
5 10.0 0.0 0.0 0.0 2.0 0.0 10.0 0.0 0.0 0.0 0.0 2.0
10

2.
DNA
2002-2005

2.
(2002)
(2004)

2.
-1
-2
-3
-4
95370_at
12 16 20 24 28 32 36 40 44 48 52 56
24

2.
-1
-2
-3
-4
95370_at
12 16 20 24 28 32 36 40 44 48 52 56
, ,
()

2.
24h
n
p
^σ
2
BIC = n log 2π + n log ˆσ 2 + n + (p + 1) log n

2.
12422(12)1000
(/×/)
1500
1125
750
375
0
52
3
3 3
50 61
330
50
3
291 307
236
904 701 716 624
DC DZ WC WZ
only 1 experiment
2 experiments
3 experiments
4 experiments
16

3.
2003-2005

CBRC
Computational Biology Research Center
National Institute of
Advanced Industrial Science and Technology
(AIST)
TOMINAGA daisuke
KADOTA Koji
TAKAHASHI Katsutoshi
Paul HORTON
RICE
Tissue Engineering Research Complex
(Research Institute for Cell Engineering)
National Institute of
Advanced Industrial Science and Technology
(AIST)
YOSHIKAWA Tomohiro
MIYAKE Masato
MIYAKE Jun
Acknowledgment
2003-2005

TFA
Transfection array
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TFA
AIST (MIT)
H17 NEDO(, , )

TFA

TFA
AIC
AIC
AIC = n log σ + √ 2s log(n!)
n
s : n :
σ :
α k =
k∑
1
c N c
∑
i,j∈Z c
p ij
pij : Zc : c
Nc : Zc

TFA
=
A
B

TFA

TFA
HeLa
(RICE)

TFA
HeLa
(RICE)
GRE AP1(PMA)
STAT3 NFAT Caspase3 TRE RARE p53-EGFP Myc GAS ISRE ERE SRE CREB-EGFP
CREB-EGFP AP1(PMA) p53-EGFP NFKB ERE p53
NFAT GAS IkB-EGFP TRE SRE Myc
ERE AP1(PMA)
p53-EGFP E2F CRE AP1 Rb TRE RARE NFAT IKB-EGFP p53 Caspase3 ISRE GRE GAS STAT3 Myc SRE HSE

TFA
HeLa

TFA
28

4.
sAnalysis
S-system
clarinet
TFA
DBGEO Web
S-system

4.
clarinet
S-system
sAnalysis

TFA
AIST
NEDO

=

=

clarinets

http://unit.aist.go.jp/terc/japanese/team/gtt/cellinfo/01_tech/tech_01.htm
TERC(2003)

H15jyuutenHoukokuFromMasato.doc

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2001 2002 2003 2004 2005
TFA
Software
Micro-array
Network model
01 02 03 04 05
TFA
Software
Micro-array
Network model
01 02 03 04 05
TFA
Software
Micro-array
Network model
01 02 03 04 05
TFA
Software
Micro-array
Network model
01 02 03 04 05

http://www.nbu.ac.jp/~yasuda/flask_tt.gif
http://blogs.osafoundation.org/blogotomy/images/macintosh-128k.jpg