Solvent UV Cutoffs

# Name RT Area[µV,Sec] %Area Plates Resolution Asymmetry Capacity
1 4,743 71337,705 0,17 7121 0,00 0,9 0,64
2 5,238 335401,895 0,80 2651 1,57 0,9 0,81
3 6,580 19340,200 0,05 13582 4,25 1,3 1,27
4 7,070 62830,166 0,15 11329 2,00 1,3 1,44
5 19,150 20563361,829 49,34 13071 25,88 1,0 5,60
6 25,343 20624625,900 49,49 12529 7,88 1,2 7,74
Total Area of Peak = 41676897.695 [µV.Sec]
Injection Volume = 20.00 ul
# Name RT Area[µV,Sec] %Area Plates Resolution Asymmetry Capacity
1 3,045 349209,909 0,78 490 0,00 1,3 0,05
2 3,805 37325,488 0,08 1252 1,55 1,1 0,31
3 4,648 137979,055 0,31 3624 2,29 0,7 0,60
4 5,032 601260,740 1,34 1303 0,89 1,8 0,74
5 6,458 58580,335 0,13 3121 2,80 0,9 1,23
6 18,293 22066054,966 49,02 1806 10,86 1,6 5,31
7 23,992 21766791,225 48,35 1920 2,92 1,6 7,27
Total Area of Peak = 45017201.718 [µV.Sec]
Injection Volume = 20.00 ul

Solvent UV Cutoffs
Solvent
UV Cutoffs
Ethylene dichloride
230 nm
Acetonitrile
190 nm
Ethyl ether
220 nm
Amyl alcohol
210 nm
Ethyl sulfide
290 nm
Acetone
330 nm
Isopropyl ether
220 nm
Amyl chloride
225 nm
Isopropyl chloride
225 nm
Benzene
280 nm
Isopropanol
205 nm
Cyclohexane
200 nm
Methanol
205 nm
Cyclopentane
200 nm
Methylene chloride
233 nm
Carbon disulfide
380 nm
Methyl-isobutyl ketone
334 nm
Carbon tetracholoride
265 nm
Methyl ethyl ketone
330 nm
Chloroform
245 nm
1-Nitropropane
380 nm
Diethyl amine
275 nm
Nitromethane
380 nm
Ethanol
210 nm
Octane
215 nm
Ethylene glycol
210 nm
n-Pentane
190 nm
2-Butoxyethanol
220 nm
Petroleum ehter
210 nm
p-Dioxane
215 nm
n-Propanol
210 nm
Ethyl acetate
256 nm
n-Propyl chloride
225 nm
Pyridine
330 nm
Toluene
285 nm
Tetrahydrofuran
230 nm
Xylene
290 nm

Wavelength Cutoffs for Common Mobile Phases
Mobile Phase
UV Cutoff
1% Acetic acid 230 nm
10 mM Ammonium bicarbonate 190 nm
0.1% BRIJ 35 190 nm
0.1% CHAPS 215 nm
50 mM Diammonium phosphate 230 nm
10 mM Ammonium acetate 205 nm
1 mM Na 2
-EDTA 190 nm
0.1% HCl 190 nm
10 mM HEPES (pH 7.6) 225 nm
10 mM MES (pH 6.0) 225 nm
10 mM Potassium phosphate-monobasic 190 nm
10 mM Potassium phosphate-dibasic 230 nm
PIC Reagent B-6 (1 vial/liter)
225 nm
PIC Reagent D-4 (1 vial/liter)
190 nm
PIC Reagent A (1 vial/liter)
< 200 nm
1 M NaCl 208 nm
10 mM Sodium citrate 225 nm
10 mM Sodium acetate 205 nm
0.1% SDS 190 nm
10 mM Sodium formate 200 nm
0.1% Trifluoroacetic acid (TFA) 205 nm
0.1% Triethylamine 235 nm
0.15 Tween 20 190 nm
0.1% Triton-X 100 240 nm
20 mM Tris-HCl (pH 7.0) 204 nm
20 mM Tris-HCl (pH 8.0) 212 nm

Al 2
O 3
SiO 2
solvent ri UV cutoff
(cP) at
20°C
Hildebrand
solubility
parameter
polarity index
(Snyder)
BP (°C)
0.01 0.01 i-octane 1.404 197-210 0.5 7 0.4 99.2
0.01 0.03 n-hexane 1.375 195 0.313 7.3 0 68.9
0.04 0.04 cyclohexane 1.427 200 0.98 8.2 0 80.7
0.04 n-decane 1.412 210 0.92 7.8 0.3 174.1
0.18 0.12 carbon tetrachloride 1.466 265 0.97 8.6 1.7 76.5
0.28 0.22 i-propyl ether 1.368 220 0.33-0.37 7.3 2.2 68.3
0.29 0.23 toluene 1.496 285 0.59 8.9 2.3 101.6
0.32 0.25 benzene 1.501 280 0.65 9.2 3 80.1
0.36-0.4 0.26 chloroform 1.443 245 0.57 9.2 3.4-4.4 61.2
0.4 0.32 dichloromethane 1.424 232 0.44 9.6 3.4 40
0.42 0.32 methylene chloride 1.424 233 0.44 9.7 3.4 39.8
0.45 0.35 tetrahydrofuran 1.408 212-230 0.55 9.1 4.2 66
0.49 0.38 ethylene dichloride 1.445 230 0.79 9.7 3.7 83.5
0.51 0.39 methyl ethyl ketone (MEK) 1.379 330 0.43 9.3 4.5 80
0.56-0.58 0.47-0.53 acetone 1.359 330 0.32 9.6 5.4 56.3
0.55-0.65 0.5 acetonitrile 1.344 190 0.37 11.7 6.2 81.6
0.58 0.38-0.48 ethyl acetate 1.37 256 0.46-0.47 9.1 4.3 77.1
0.6 0.46 methyl acetate 1.362 260 0.37-0.45 9.2 4.4 56.3
0.62 0.41 dimethyl sulfoxide 1.478 2.24 12.8 6.5 189
0.71 pyridine 1.51 305-330 0.94 10.7 5.3 115.3
0.74 methoxyethanol, 2- 1.401 220 1.72 5.7 124.6
0.82 propanol, 2- 1.38 210+ 2.35 4.3 82.4-117.7
0.88 0.68 ethanol 1.361 205-210 1.2 12 5.2 78.3
0.95 0.73 methanol 1.329 205 0.6 13.7 6.6 64.7
large acetic acid 1.372 210 1.1-1.26 12.4 6.2 117.9
large water 1.333 180 1 21 9 100

polarity
index
(Snyder)
solvent
ri
UV
cutoff
(cP)
at 20°C
Hildebrand
solubility
parameter
SiO 2
Al 2
O 3
BP (°C)
0 cyclohexane 1.427 200 0.98 8.2 0.04 0.04 80.7
0 n-hexane 1.375 195 0.313 7.3 0.03 0.01 68.9
0.3 n-decane 1.412 210 0.92 7.8 0.04 174.1
0.4 i-octane 1.404
197-
210
0.5 7 0.01 0.01 99.2
0.4 octane 1.395 0.5 99.2
1.7 butyl ether 0.7 142.2
1.7 carbon tetrachloride 1.466 265 0.97 8.6 0.12 0.18 76.5
1.8 triethyl amine 1.401 0.38 89.5
2.2 i-propyl ether 1.368 220
0.33-
0.37
7.3 0.22 0.28 68.3
2.3 toluene 1.496 285 0.59 8.9 0.23 0.29 101.6
2.4 xylene, p- 0.7 138
2.9 t-butyl methyl ether 1.370 210 0.27 0.35 55.2
3 benzene 1.501 280 0.65 9.2 0.25 0.32 80.1
3.3 benzyl ether 5.33 288.3
3.4 dichloromethane 1.424 232 0.44 9.6 0.32 0.4 40
3.4 methylene chloride 1.424 233 0.44 9.7 0.32 0.42 39.8
3.4-4.4 chloroform 1.443 245 0.57 9.2 0.26 0.36-0.4 61.2
3.7 dichloroethane 83.4
3.7 ethylene dichloride 1.445 230 0.79 9.7 0.38 0.49 83.5
3.9 butanol, 1- 117.2
3.9 i-butyl alcohol 3 117.7
4.2 tetrahydrofuran 1.408
212-
230
0.55 9.1 0.35 0.45 66

polarity
index
(Snyder)
solvent ri UV cutoff
(cP) at
20°C
Hildebrand
solubility
parameter
SiO 2
Al 2
O 3
BP (°C)
4.3 ethyl acetate 1.370 256
0.46-
0.47
9.1
0.38-
0.48
0.58 77.1
4.3 propanol, 1- 2.3 97.2
4.3 propanol, 2- 1.380 210+ 2.35 0.82
82.4-
117.7
4.4 methyl acetate 1.362 260
0.37-
0.45
9.2 0.46 0.6 56.3
4.5 cyclohexanone 2.24 155.7
4.5 methyl ethyl ketone (MEK) 1.379 330 0.43 9.3 0.39 0.51 80
4.5 nitrobenzene 2.03 210.8
4.6 benzonitrile 1.22 191.1
4.8 dioxane, 1,4- 101
4.8 dioxane, p 1.54 101.3
5.2 ethanol 1.361
205-
210
1.2 12 0.68 0.88 78.3
5.3 nitroethane 1.392 380 0.68 114
5.3 pyridine 1.510
305-
330
0.94 10.7 0.71 115.3
5.4 acetone 1.359 330 0.32 9.6
0.47-
0.53
0.56-
0.58
56.3
5.5 benzyl alcohol 5.8 205.5
5.7 methoxyethanol, 2- 1.401 220 1.72 0.74 124.6
6.2 acetic acid 1.372 210 1.1-1.26 12.4 large 117.9
6.2 acetonitrile 1.344 190 0.37 11.7 0.5
0.55-
0.65
81.6
6.4 dimethyl formamide, N,N- 1.431 268
0.90-
0.92
11.5 153
6.5 dimethyl sulfoxide 1.478 2.24 12.8 0.41 0.62 189
6.6 methanol 1.329 205 0.6 13.7 0.73 0.95 64.7
7.3 formamide 1.450 210 3.3-3.76 210.5
9 water 1.333 180 1 21 large 100

solvent
ri
UV
cutoff
(cP)
at 20°C
Hildebrand
solubility
parameter
2
SiO
3
Al 2
O
polarity
index
(Snyder)
BP (°C)
acetaldehyde diethyl
acetal
1.3
79
acetaldehyde diethyl
acetal
1.3
79
acetic acid
acetic anhydride
1.3
72
1.3
89
210 1.1-1.26 12.4 large 6.2 117.9
acetone
1.3
59
330 0.32 9.6
0.47-
0.53
0.56-0.58 5.4 56.3
acetonitrile
benzene
1.3
44
1.5
01
190 0.37 11.7 0.5 0.55-0.65 6.2 81.6
280 0.65 9.2 0.25 0.32 3 80.1
benzonitrile 1.22 4.6 191.1
benzyl alcohol 5.8 5.5 205.5
benzyl ether 5.33 3.3 288.3
butanol, 1- 3.9 117.2
butanol, 2-
butanone, 2-
butyl acetate
butyl acetate, sec-
1.3
95
1.3
77
1.3
92
1.3
87

Solvent
ri
UV
cutoff
(cP)
at 20°C
Hildebrand
solubility
parameter
2
SiO
3
Al 2
O
polarity
index
(Snyder)
BP (°C)
butyl ether 0.7 1.7 142.2
butyl ethyl ether
butyl formate
butylamine, 2-
butyraldehyde
butyric acid
butyronitrile
carbon tetrachloride
chlorobutane, 2-
chloroform
chloropropane, 1-
chloropropane, 2-
cyclohexane
1.3
80
1.3
87
1.3
90
1.3
78
1.3
96
1.3
82
1.4
66
1.3
95
1.4
43
1.3
86
1.3
76
1.4
27
265 0.97 8.6 0.12 0.18 1.7 76.5
245 0.57 9.2 0.26 0.36-0.4 3.4-4.4 61.2
200 0.98 8.2 0.04 0.04 0 80.7
cyclohexanone 2.24 4.5 155.7

Solvent
ri
UV
cutoff
(cP)
at 20°C
Hildebrand
solubility
parameter
2
SiO
3
Al 2
O
polarity
index
(Snyder)
BP (°C)
dichloroethane 3.7 83.4
dichloromethane
diethyl carbonate
di-isopropylamine
1.4
24
1.3
85
1.3
90
232 0.44 9.6 0.32 0.4 3.4 40
1.4
31
268
dimethyl formamide, N,N-
0.90-
0.92
11.5 6.4 153
dimethyl sulfoxide
dimethylbutane, 2,2-
dimethylbutane, 2,3-
dimethylpentane, 2,3-
dimethylpentane, 2,4-
1.4
78
1.3
69
1.3
72
1.3
89
1.3
79
2.24 12.8 0.41 0.62 6.5 189
dioxane, 1,4- 4.8 101
dioxane, p 1.54 4.8 101.3
dodecafluoro-1-hepatanol
1.3
16
ethanol
1.3
61
205-
210
1.2 12 0.68 0.88 5.2 78.3

Solvent
ri
UV
cutoff
(cP)
at 20°C
Hildebrand
solubility
parameter
2
SiO
3
Al 2
O
polarity
index
(Snyder)
BP (°C)
ethyl acetate
1.3
70
256
0.46-
0.47
9.1
0.38-
0.48
0.58 4.3 77.1
ethyl ether
1.3
52
ethyl formate
1.3
58
ethyl propionate
1.3
82
ethylene dichloride
1.4
45
230 0.79 9.7 0.38 0.49 3.7 83.5
formamide
1.4
50
210 3.3-3.76
pyridine
1.5
10
305-
330
0.94 10.7 0.71 5.3 115.3
t-butanol
1.3
85
t-butyl methyl ether
1.3
70
210 0.27 0.35 2.9 55.2
tetrahydrofuran
1.4
08
212-
230
0.55 9.1 0.35 0.45 4.2 66
toluene
triethyl amine
trifluoroacetic acid
1.4
96
1.4
01
1.2
83
285 0.59 8.9 0.23 0.29 2.3 101.6
0.38 1.8 89.5

Solvent
ri
UV
cutoff
(cP)
at 20°C
Hildebrand
solubility
parameter
2
SiO
3
Al 2
O
polarity
index
(Snyder)
BP (°C)
trifluoroethanol
1.2
90
trifluoropropanol
1.3
81
trimethylbutane, 2,2,3-
1.3
87
trimethylhexane, 2,2,5-
1.3
97
trimethylpentane, 2,2,4-
1.3
89
valeronitrile
1.3
95
water
1.3
33
180 1 21 large 9 100
xylene, p- 0.7 2.4 138

COLUMN DIMENSIONS (I.D. x L(mm))
VOID VOLUME (ml)
1.0 x 100 0.06
1.0 x 150 0.08
1.0 x 250 0.14
1.0 x 300 0.17
2.1 x 100 0.24
2.1 x 150 0.37
2.1 x 250 0.61
2.1 x 300 0.73
4.6 x 100 1.16
4.6 x 150 1.75
4.6 x 250 2.90
4.6 x 300 3.49
7.8 x 100 3.35
7.8 x 150 5.02
7.8 x 250 8.36
7.8 x 300 10.04
10.0 x 100 5.50
10.0 x 150 8.25
10.0 x 250 13.75
10.0 x 300 16.49
21.0 x 100 24.25
21.0 x 150 36.37
21.0 x 250 60.61
21.0 x 300 72.74
50.0 x 100 137.45
50.0 x 150 206.17
50.0 x 250 343.61
50.0 x 300 412.33
* Note : Assumes an Average Pore Volume of 0.70.
Void Volume (ml) = d^2 *Pi * L * Pore Volume / 4

dp
(µm)
ID
(mm)
L
(mm)
typical flow
(mL/min)
typical pressure* N t o
(mL) sample load
(mg)**
(psi) (bar)
sample volume
(µL)
5 1 150 0.1 1500 103.4 - - ~0.05 0.05-1
5 2 150 0.2 750 51.7 - - - -
3 3 150 0.42 1586 109.4 12500 0.85 -- -
4 3 150 0.42 892 61.5 9375 0.85 -- -
5 3 150 0.42 571 39.4 7500 0.85 -- -
5 3.2 150 0.48 571 39.4 7500 0.97 -- -
3 4.6 150 0.5 985 68.0 - 1.99 - -
5 4.6 150 1 571 39.4 7500 1.99 -- -
10 4.6 150 2 355 24.5 - 1.99 - -
5 10 150 5 750 51.7 - - - -
10 21.2 150 20 170 11.7 - - - -
5 1 250 0.1 2500 172.4 - - - -
5 2 250 0.2 1250 86.2 - - 0.2 0.2-5
5 3 250 0.42 952 65.6 12500 1.41 -- -
5 3.2 250 0.48 952 65.6 12500 1.61 1 1-20
3 4.6 250 0.5 1640 113.1 - 3.32 - -
5 4.6 250 1 1180 81.4 12500 3.32 5 5-80
10 4.6 250 2 590 40.7 - - - -
5 10 250 5 1250 86.2 - 9.82 30 30-500
10 21.2 250 20 280 19.3 - - - -
* typical values for water as eluens
** dependant on sample composition