0-TESTO COMPLETO.pdf - Fondazione Santa Lucia

Sezione III: Attività per progetti
with PBS and incubated overnight with 1 ml of TSB broth containing antibiotic
in a concentration higher than its MIC. Polymer disks will then be
washed twice with PBS to remove planktonic cells, and CFU values will be
enumerated by sonication, vortex agitation and dilution plating as described
above.
Expected results: Polymers loaded with Dispersin B plus Vancomycin will
resist colonization and biofilm formation more efficiently that polymers
loaded with Dispersin B or antimicrobial agent alone.
POTENTIAL PROBLEMS
Dispersin B may not sensitize bacteria to killing by Vancomycin. In this
case an other antibiotic (Tygecycline or Daptomycin) will be tested.
Dispersin B may not sensitize S. aureus biofilms to killing by Vancomycin,
possibly due to the small amount of PNAG present in S. aureus
biofilms [Izano et al. 2008a]. In this case, an alternative matrix-degrading
enzyme such as DNase I could be employed.
Novel polymers may not adsorb Vancomycin. In this case, we may vary
the polymer chemical features according to Vancomycin properties, or
increase the concentration of functional groups per polymer repetitive unit so
that a higher number of antibiotic molecules could be coordinated by the
polymer.
Dispersin B and Vancomycin may be released too rapidly from the loaded
polymers. This problem could be overcome by incorporating pore-forming
molecules such as albumin or polyethylenglycol into the polymer in order to
modulate antibiotic desorption [Donelli et al. 2006; Ruggeri et al. 2007].
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800 2009