0-TESTO COMPLETO.pdf - Fondazione Santa Lucia

Sezione III: Attività per progetti
vehicle. Brains from a subset of animals will be processed for determination
of the volume and area of NMDA-injured cortex, using an image analyzer.
The remaining animals will be transcardially perfused and processed for
immunohistochemistry [Giampà et al. 2006], which will be performed on cortices
to evaluate BDNF immunoreactivity. Immunolabeled tissue will be
observed under a fluorescence microscope and images will be digitally
acquired to evaluate possible labeling intensity differences between treatments.
These experiments will show if the PDE inhibitor TP-10 exerts a direct
neuroprotective role in the cortical neurodegeneration model of NMDA
lesion, and if neuroprotection is comparable to the one achieved by PDE4
inhibitor rolipram [DeMarch et al. 2007]. Moreover, the BDNF immunolabeling
studies will show if neuroprotection is linked to the upregulation of
BDNF. That will shed light on the possibility of using PDE inhibitors in neurodegenerative
disorders where cortex is involved, such as AD.
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670 2009