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0-TESTO COMPLETO.pdf - Fondazione Santa Lucia

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ISS.17 – Biomarkers and diagnosis<br />

matory cytokines will then be measured via intracellular staining or ELISA.<br />

Moreover, the frequency of cells which bind the FITC-conjugated pentamers<br />

will also be measured, and polychromatic flow cytometry will be used to characterize<br />

these cells. Finally, pentamer + cells will be sorted and expanded in<br />

vitro to be further characterized (cytoxicity assays using B-EBV targets).<br />

The frequency of T reg cells will also be measured, and their phenotype<br />

will be studied in detail. Functional assays will evaluate their suppressive abilities<br />

and their role in the control of EBV-specific T cells.<br />

2009 647

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