universita' degli studi di milano - Alessandro Guerini Rocco

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UNIVERSITA’ DEGLI STUDI DI MILANO
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INDICE Pagina INTRODUZIONE 1 1) Est
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3) Western blot Procedimento 93 93
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INTRODUZIONE GLI ESTROGENI E I LORO
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Estrogeni e progesterone sono i pri
Page 11 and 12:
Struttura dei recettori degli estro
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A/B C D E F DBD LBD AF-1 AF-2 NLS d
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hERα hERβ N N AF-1 DNA LIGANDO C
Page 17 and 18:
hanno un grado maggiore di omologia
Page 19 and 20:
MECCANISMO D’AZIONE DEGLI ESTROGE
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A C E 2 E 2 nucleo nucleo E 2 R R i
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Fattori di modificazione degli isto
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sequenze similari al NR-box. Anche
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dell’IGF-I è mediata dalla PI3K
Page 29 and 30:
mediata dall'interazione con altri
Page 31 and 32:
ER e PI3K Uno degli effetti rapidi
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hanno proposto che i recettori di m
Page 35 and 36:
INFIAMMAZIONE L’infiammazione, o
Page 37 and 38:
anaerobica essi provocano nel terri
Page 39 and 40:
le componenti dell'essudato, come l
Page 41 and 42:
vacuolo attorno alla particella est
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secreti. Le fonti cellulari più co
Page 45 and 46:
Il PAF è coinvolto nella genesi di
Page 47 and 48:
Il tumor necrosis factor α (TNFα)
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alchilazione del DNA dovuta ad alte
Page 51 and 52:
Struttura 3D dell'eterodimero p65/p
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C-terminale, detta TA1, e una di 90
Page 55 and 56:
Delezioni o mutazioni nelle sequenz
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Sono anche presenti regioni ricche
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di CREB binding protein (p300/CBP)
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complesso nel citoplasma [200, 201]
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In diversi studi è stato messo in
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NF-κB ed LPS L’endotossina batte
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IkBα IKKα NF-κB NF-κB kB-site L
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Estrogeni e patologie a componente
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cerebellari (mancanza di coordinazi
Page 73 and 74:
L’EAE può essere indotta, in cep
Page 75 and 76:
La leucodistrofia a cellule globoid
Page 77 and 78:
L’artrite reumatoide L’artrite
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L’ischemia cerebrale Con il termi
Page 81 and 82:
dimostrato un aumento dell’espres
Page 83 and 84:
taglio è generato un Aβ di 40 o d
Page 85 and 86:
d’inibire l’attivazione della m
Page 87 and 88:
umano, hanno mostrato che il 17β-e
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SCOPO DELLA TESI Lo scopo di questa
Page 91 and 92: suddivise due volte la settimana in
Page 93 and 94: Procedimento 1. Sciogliere la polve
Page 95 and 96: STRIPAGGIO DEL SIERO DCC 24 ore pri
Page 97 and 98: Soluzioni Lysis buffer per estratti
Page 99 and 100: piastre da 96 pozzetti (Costar) in
Page 101 and 102: solution. Come anticorpo secondario
Page 103 and 104: Laemmli buffer 3X Tris pH 6,8 150 m
Page 105 and 106: Immunodetezione: 1. Togliere il PBS
Page 107 and 108: Soluzione di 3,3’-diaminobenzidin
Page 109 and 110: certezza che l'anticorpo riconosca
Page 111 and 112: 2. Azione del 17β-estradiolo sulla
Page 113 and 114: 3. Effetto del 17β-estradiolo sull
Page 115 and 116: 4. Influenza del 17β-estradiolo su
Page 121 and 122: 7. Azione del 17β-estradiolo su al
Page 123 and 124: p50 Densità ottica p50/β-actina C
Page 125 and 126: p65 citoplasm. (% cell pos /cell to
Page 127 and 128: DISCUSSIONE Numerosi studi indicano
Page 129 and 130: BIBLIOGRAFIA 1. Barnea, E.R., N.J.
Page 131 and 132: 33. Pike, A.C.W., A.M. Brzozowski,
Page 133 and 134: 63. Ignar-Trowbridge, D.M., M. Pime
Page 135 and 136: signaling in the cardiovascular sys
Page 137 and 138: to activation of the mitogen-activa
Page 139 and 140: 153. Firestein, G.S., The T cell co
Page 141: 185. Wang, D. and A.S. Baldwin, Act
Page 145 and 146: 251. Wluka, A.E., F.M. Cicuttini, a
Page 147 and 148: estradiol-mediated protection again
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