N. Bresolin - E. Medea
N. Bresolin - E. Medea
N. Bresolin - E. Medea
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Autologous transplantation of muscle-derived AC133+<br />
cells<br />
Tibialis Anterior muscle (1gr)<br />
Muscle dissotiation LIBERASE Hi<br />
Left abductor digiti minimi muscle (ADM)<br />
Quality control,<br />
microbiology<br />
Myofibers Injections of 20X103AC133+ cells<br />
*15ml Hamilton with a 27-G needle<br />
Injection site<br />
•RPMI +<br />
In vitro serum free<br />
culture for 48h<br />
•human albumin 20%+<br />
•human insulin 100 Ul/m<br />
*5ml of cell suspension delivered in<br />
each injection<br />
*Injection depth 0.5 cm,interinjection<br />
distances 1mm (sterile<br />
transparent grid)
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