Special topic: Metagenomics - Genome Sciences

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Faster, cheaper DNA sequencing Today, DNA sequencing costs 1/10,000th of what it used to. Impact on biology: • Human genetic variation • <strong>Metagenomics</strong>: • Environmental • Organismal transistors per chip bp sequenced per person/day Bio-ERA, 2007
16s rRNA: Molecular clocks & evolutionary trajectories DNA sequence similarity provides evolutionary relationships between organisms (phylogeny). What makes a good molecular clock? ‣ No strong selective forces. ‣ Ubiquitous ‣ Large: provides sufficient information. ‣ 16s rRNA sequence similarity of 97% is generally defined as a species (ribotype or phylotype). 1 E ~~~~~~~~~~~~~~~~~~~~~ 1: 2: 3: 4: 2 3 A A C C G G T T A A C C G G T G A C C C G G T C A C C C G G T A 4 Woese, 1987
Page 1 and 2: MetaGenomics Carlos Araya | 2.6.08
Page 3: What is metagenomics? (and why now?
Page 7 and 8: How complex are different environme
Page 9 and 10: percent similarity 50 100 Bible: Fr
Page 11 and 12: Yooseph et al., 2007
Page 13 and 14: Ocean’s diversity landscape is fa
Page 15 and 16: How much sequencing does it take? #
Page 17 and 18: # reads required fraction covered
Page 19 and 20: Sequencing of entire BAC insert Gen
Page 21 and 22: Gene prediction: Clustering ≥3 fi
Page 23 and 24: Sorcerer II: What Gentle lysis, did
Page 25 and 26: grouping shows that our current GOS
Page 27 and 28: Sargasso Sea North Atlantic Coast 1
Page 29 and 30: and phosphatases protein kinases se
Page 31 and 32: A few words on Craig Venter…
Page 33 and 34: of this yloonnearrmiinoare S1. ject
Page 35 and 36: “The best-laid plans of mice & me
Page 37 and 38: Computational: ‣ Genome assembly
Page 39 and 40: Biocatalysis Bio-catalysis: Greener
Page 41 and 42: TTG-8 [2] TTG-9 [5] TTG-10 [39] Bio
Page 44 and 45: Woese, 1987

Special topic: Metagenomics - Genome Sciences

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