Special topic: Metagenomics - Genome Sciences
Special topic: Metagenomics - Genome Sciences
Special topic: Metagenomics - Genome Sciences
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Reads<br />
<strong>Genome</strong> coverage (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
10000000<br />
40<br />
9000000<br />
30<br />
20<br />
8000000<br />
10<br />
7000000<br />
6000000<br />
5000000<br />
4000000<br />
0<br />
3000000<br />
2000000<br />
1000000<br />
0<br />
1<br />
1<br />
6<br />
7<br />
<strong>Genome</strong>-centric vs. Gene-centric views<br />
11<br />
13<br />
16<br />
19<br />
21<br />
26<br />
25<br />
31<br />
31<br />
36<br />
37<br />
41<br />
43<br />
46<br />
49<br />
51<br />
55<br />
56<br />
61<br />
Species abundance (%)<br />
61<br />
66<br />
Species abundance (%)<br />
67<br />
71<br />
73<br />
76<br />
1x coverage<br />
5x coverage<br />
10x coverage<br />
79<br />
81<br />
85<br />
86<br />
91<br />
91<br />
96<br />
97<br />
For a species 10% abundant, 10x<br />
library coverage:<br />
‣ 1 million sequencing reads<br />
‣ Cover 63% of the genome<br />
Ouch.
Change language