Special topic: Metagenomics - Genome Sciences

s
-
c
,
-
s
-
-
t
r
t
-
b
Percentage of total
sequences
c
Microbial T3 ecology alters with weight-loss
T2
Dieting alters the balance between Bacteriodetes and Firmicutes in humans.
Bacteriodetes R enrichment is independent of diet type: carb-restricted or fatrestricted.
2 = 0.8
R2 30 CARB-R
Figure 1 25|
Correlation FAT-Rbetween
body-weight loss and gut microbial ecology. a, Clustering of 16S
ribosomal RNA gene sequence libraries of faecal microbiota for each person (in different colours)
and time
20
point in diet therapy (T0, baseline; T1, 12 weeks; T2, 26 weeks; T3, 52 weeks) in the two diettreatment
15 groups (fat restricted, FAT-R; carbohydrate restricted, CARB-R), based on UniFrac analysis
of the 18,348-sequence phylogenetic tree. b, Relative abundance of Bacteroidetes and Firmicutes. For
10
each time point, values from all available = 0.5 samples were averaged (n was 11 or 12 per time point). Leansubject
controls 5 include four stool samples from two people taken 1 year apart, plus three other stool
samples
0
6 . Mean values ± s.e. are plotted. c, Change in relative abundance of Bacteroidetes in subjects
with weight loss above a threshold of 2% weight loss for the CARB-R diet and 6% for the FAT-R diet.
Change in Bacteroidetes
abundance (%)
100
80
60
40
20
FAT-R
T1
T0
T1
T2
T3
T1
T2
T1
T3
T0
T3
T0
T2
T1
T3
T0
T1
T2
T3
Firmicutes Bacteroidetes
CARB-R
0 12 26 52 Lean
Weeks on diet
T1
T0
T1
T1
T2
T0
T2
T3
T1
T2
T3
T1
T1
T3
T3
T0
T0
Percent
seq
c
Change in Bacteroidetes
abundance (%)
40
20
30 CARB-R
25
FAT-R
20
15
10
5
0
0
0 12 26 52 Lean
Weeks on diet
R 2 = 0.8
R 2 = 0.5
-5 -10 -15 -20
Change in body weight (%)
-25
Ley et al., 2006